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首页> 外文期刊>Journal of Experimental Botany >CEPR2 phosphorylates and accelerates the degradation of PYR/PYLs in Arabidopsis
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CEPR2 phosphorylates and accelerates the degradation of PYR/PYLs in Arabidopsis

机译：CEPR2磷酸化并加速拟南芥中Pyr / Pyls的降解

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摘要

Pyrabactin resistance 1 (PYR1)/PYR1-like (PYL) abscisic acid (ABA) receptors have been proved to be recruited in the plasma membrane (PM). In order to explain the roles of PYR/PYLs in the PM, PYL4 was used as bait to screen the PM-localized leucine-rich repeat receptor-like kinase family, and five members were found directly interacting with PYL4. Loss of function by T-DNA insertion in C-terminally encoded peptide receptor 2 (CEPR2) together with phloem intercalated with xylem (PXY) and PXY-Like 2 (PXL2) led to ABA hypersensitivity, while CEPR2 overexpression led to ABA insensitivity compared with the wild type, indicating the redundant and negative roles of CEPR2, PXY, and PXL2 in ABA signaling. The PYL4 proteins were strongly accumulated in cepr2/pxy/pxl2 compared with the wild type. Furthermore, higher phosphorylation levels accompanied by lower protein levels of PYL4 in CEPR2 overexpression lines were observed, indicating the requirement of phosphorylation of PYLs for degradation. Subsequently, MS and in vitro kinase assays demonstrated that CEPR2 phosphorylated PYL4 at Ser54, while this phosphorylation was diminished or even eliminated in the presence of ABA. Taken together, CEPR2 promotes the phosphorylation and degradation of PYLs in unstressed conditions, whereas ABA represses this process to initiate ABA response during times of stress.
机译：已证明在血浆膜（PM）中募集丙比肌菌素抗性1（PYR1）/ PYR1样（Pyl）脱落酸（ABA）受体。为了解释PYR / PYLS在PM中的作用，Pyl4用作诱饵以筛选PM局部化的富含亮氨酸的重复受体样激酶家族，并且发现五个成员直接与Pyl4相互作用。通过将C末端编码的肽受体2（CEPR2）中的T-DNA插入与用木质（PXY）和PXY样2（PXL2）相插的韧皮腔内的功能丧失，LED为ABA超敏反应，而CEPR2过度表达导致ABA绝积与野生类型，表明CEPR2，PXY和PXL2在ABA信令中的冗余和负角色。与野生型相比，Pyl4蛋白在CEPR2 / PXY / PXL2中强烈累积。此外，观察到CePR2过表达系中Pyl4伴随蛋白质水平的较高的磷酸化水平，表明Perss磷酸化以降解。随后，MS和体外激酶测定证明了SER54的CEPR2磷酸化Pyl4，同时在ABA存在下降低或甚至消除该磷酸化。 CePR2一起携带CePR2在不重定相位的条件下促进Pyls的磷酸化和降解，而ABA抑制该过程以在压力期间引发ABA响应。

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  • 来源
    《Journal of Experimental Botany》 |2019年第19期|共13页
  • 作者

    Yu Zipeng; Zhang Di; Xu Yang; Jin Songsong; Zhang Lei; Zhang Shizhong; Yang Guodong; Huang Jinguang; Yan Kang; Wu Changai; Zheng Chengchao;

  • 作者单位

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

    Shandong Agr Univ Coll Life Sci State Key Lab Crop Biol Tai An 271018 Shandong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    Abscisic acid; CEPR2; degradation; phosphorylation; plasma membrane; PYR/PYLs;

    机译：脱落酸;CEPR2;降解;磷酸化;血浆膜;Pyr / Pyls;

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