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首页> 外文期刊>Journal of Experimental Botany >jaw-1D: a gain-of-function mutation responsive to paramutation-like induction of epigenetic silencing
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jaw-1D: a gain-of-function mutation responsive to paramutation-like induction of epigenetic silencing

机译：jaw-1d：响应于载气诱导的函数诱导的功能突变

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摘要

The Arabidopsis thaliana gain-of-function T-DNA insertion mutant jaw-1D produces miR319A, a microRNA that represses genes encoding CIN-like TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTORs (TCPs), a family of transcription factors that play key roles in leaf morphogenesis. In this study, we show that jaw-1D is responsive to paramutation-like epigenetic silencing. A genetic cross of jaw-1D with the polycomb gene mutant curly leaf-29 (clf-29) leads to attenuation of the jaw-1D mutant plant phenotype. This induced mutation, jaw-1D*, was associated with down-regulation of miR319A, was heritable independently from clf-29, and displayed paramutation-like non-Mendelian inheritance. Down-regulation of miR319A in jaw-1D* was linked to elevated levels of histone H3 lysine 9 dimethylation and DNA methylation at the CaMV35S enhancer located within the activation-tagging T-DNA of the jaw-1D locus. Examination of 21 independent T-DNA insertion mutant lines revealed that 11 could attenuate the jaw-1D mutant phenotype in a similar way to the paramutation induced by clf-29. These paramutagenic mutant lines shared the common feature that their T-DNA insertion was present as multi-copy tandem repeats and contained high levels of CG and CHG methylation. Our results provide important insights into paramutation- like epigenetic silencing, and caution against the use of jaw-1D in genetic interaction studies.
机译：拟南芥拟南芥的功能性T-DNA插入突变体JAW-1D产生miR319a，一种microRNA，抑制编码Cin样Teosinte分支的基因的基因，这是一种转录因子的一系列转录因子，可在其中发挥关键作用叶形态发生。在这项研究中，我们表明JAW-1D对令人敏锐的偏心沉默响应。具有Polycomb基因突变体卷曲叶片-99（CLF-29）的jaw-1d的遗传交叉导致jaw-1d突变植物表型的衰减。这种诱导的突变jaw-1d *与miR319a的下调有关，与CLF-29独立地遗传，并且显示出类似的非孟德利亚遗传。在jaw-1d *中miR319a的下调与位于Jaw-1d基因座的活化标签T-DNA内的CAMV35S增强剂处的组蛋白H3赖氨酸9升高和DNA甲基化的升高。检查21个独立的T-DNA插入突变体系显示，11可以以与CLF-29引起的载气相同的方式衰减JAW-1D突变表型。这些Paramutagenic突变线共享其作为多拷贝串联重复并包含高水平的CG和CHG甲基化的常见特征。我们的结果提供了对诸如表观遗传沉默的重要见解，并针对在遗传相互作用研究中使用JAW-1D的谨慎态度。

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  • 来源
    《Journal of Experimental Botany》 |2019年第2期|共10页
  • 作者

    Jiang Wen; Li Zhongfei; Yao Xiaozhen; Zheng Binglian; Shen Wen-Hui; Dong Aiwu;

  • 作者单位

    Fudan Univ CNRS Inst Plant Biol Sch Life Sci Int Associated Lab Fudan HUNAU Plant Collaborat Innovat Ctr Genet &

    Dev State Key Lab Shanghai 200438 Peoples R China;

    Fudan Univ CNRS Inst Plant Biol Sch Life Sci Int Associated Lab Fudan HUNAU Plant Collaborat Innovat Ctr Genet &

    Dev State Key Lab Shanghai 200438 Peoples R China;

    Fudan Univ CNRS Inst Plant Biol Sch Life Sci Int Associated Lab Fudan HUNAU Plant Collaborat Innovat Ctr Genet &

    Dev State Key Lab Shanghai 200438 Peoples R China;

    Fudan Univ State Key Lab Genet Engn Inst Plant Biol Collaborat Innovat Ctr Genet &

    Dev Sch Life Sci Shanghai 200438 Peoples R China;

    Fudan Univ CNRS Inst Plant Biol Sch Life Sci Int Associated Lab Fudan HUNAU Plant Collaborat Innovat Ctr Genet &

    Dev State Key Lab Shanghai 200438 Peoples R China;

    Fudan Univ CNRS Inst Plant Biol Sch Life Sci Int Associated Lab Fudan HUNAU Plant Collaborat Innovat Ctr Genet &

    Dev State Key Lab Shanghai 200438 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    Arabidopsis thaliana; DNA methylation; epigenetic silencing; histone methylation; jaw-1D; paramutation;

    机译：拟南芥天然;DNA甲基化;表观遗传沉默;组蛋白甲基化;jaw-1d;载气;

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