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首页> 外文期刊>Journal of Experimental Botany >NF-YC12 is a key multi-functional regulator of accumulation of seed storage substances in rice
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NF-YC12 is a key multi-functional regulator of accumulation of seed storage substances in rice

机译：NF-YC12是水稻种子储能物质积累的关键多功能调节器

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Starch and storage proteins, the primary storage substances of cereal endosperm, are a major source of food for humans. However, the transcriptional regulatory networks of the synthesis and accumulation of storage substances remain largely unknown. Here, we identified a rice endosperm-specific gene, NF-YC12, that encodes a putative nuclear factor-Y transcription factor subunit C. NF-YC12 is expressed in the aleurone layer and starchy endosperm during grain development. Knockout of NF-YC12 significantly decreased grain weight as well as altering starch and protein accumulation and starch granule formation. RNA-sequencing analysis revealed that in the nf-yc12 mutant genes related to starch biosynthesis and the metabolism of energy reserves were enriched in the down-regulated category. In addition, starch and protein contents in seeds differed between NF-YC12-overexpression lines and the wild-type. NF-YC12 was found to interact with NF-YB1. ChIP-qPCR and yeast one-hybrid assays showed that NF-YC12 regulated the rice sucrose transporter OsSUT1 in coordination with NF-YB1 in the aleurone layer. In addition, NF-YC12 was directly bound to the promoters of FLO6 (FLOURY ENDOSPERM6) and OsGS1;3 (glutamine synthetase1) in developing endosperm. This study demonstrates a transcriptional regulatory network involving NF-YC12, which coordinates multiple pathways to regulate endosperm development and the accumulation of storage substances in rice seeds.
机译：淀粉和储存蛋白质，谷物胚乳的主要储存物质是人类食物的主要来源。然而，储存物质的合成和积累的转录调节网络仍然很大程度上是未知的。这里，我们鉴定了一种特异性胚乳特异性基因NF-YC12，其编码推定的核因子-Y转录因子亚基C.NF-YC12在籽粒发育期间在阿列酮层和淀粉胚乳中表达。 NF-YC12的敲除显着降低了晶粒重量以及改变淀粉和蛋白质积累和淀粉颗粒形成。 RNA测序分析表明，在与淀粉生物合成相关的NF-YC12突变基因中，能量储备的代谢富集在下调类别中。此外，种子中的淀粉和蛋白质含量不同，NF-YC12过表达线和野生型不同。发现NF-YC12与NF-YB1相互作用。 CHIP-QPCR和酵母单杂化测定表明，NF-YC12在含有NF-YB1中的NF-YB1在阿仑酮层中调节水稻蔗糖转运蛋白。此外，NF-YC12直接与FLO6（泛黄胚乳植物体6）和OSGS1; 3（谷氨酰胺合成酶1）的促进剂结合。该研究表明，涉及NF-YC12的转录调节网络，其协调多种途径，以调节胚乳的开发和稻米储存物质的积累。

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