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首页> 外文期刊>Journal of Experimental Botany >The contribution of cis- and trans-acting variants to gene regulation in wild and domesticated barley under cold stress and control conditions
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The contribution of cis- and trans-acting variants to gene regulation in wild and domesticated barley under cold stress and control conditions

机译：CIS-和反式作用变体在冷应力和控制条件下在野生和驯养大麦的基因调节中的贡献

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Barley, like other crops, has experienced a series of genetic changes that have impacted its architecture and growth habit to suit the needs of humans, termed the domestication syndrome. Domestication also resulted in a concomitant bottleneck that reduced sequence diversity in genes and regulatory regions. Little is known about regulatory changes resulting from domestication in barley. We used RNA sequencing to examine allele-specific expression in hybrids between wild and domesticated barley. Our results show that most genes have conserved regulation. In contrast to studies of allele-specific expression in interspecific hybrids, we find almost a complete absence of trans effects. We also find that cis regulation is largely stable in response to short-term cold stress. Our study has practical implications for crop improvement using wild relatives. Genes regulated in cis are more likely to be expressed in a new genetic background at the same level as in their native background.
机译：像其他作物一样，大麦经历了一系列影响其建筑和增长习惯，以适应人类的需求，称为驯化综合症。 驯化也导致伴随的瓶颈减少了基因和监管区域的序列多样性。 关于大麦中的驯化导致的监管变化很少。 我们使用RNA测序来检查野生和驯养大麦的杂种中的等位基因特异性表达。 我们的结果表明，大多数基因都有守恒的监管。 相反，与三种杂种中的等位基因特异性表达的研究，我们发现几乎完全没有反式效应。 我们发现CIS调节响应短期冷压力而在很大稳定。 我们的研究对使用野生亲属进行作物改善具有实际影响。 在CIS中调节的基因更容易在与其本地背景中的相同水平的新遗传背景中表达。

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