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首页> 外文期刊>Journal of Experimental Botany >PacCYP707A2 negatively regulates cherry fruit ripening while PacCYP707A1 mediates drought tolerance
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PacCYP707A2 negatively regulates cherry fruit ripening while PacCYP707A1 mediates drought tolerance

机译：PACCYP707A2负调节樱桃果实成熟，而PACCYP707A1介导干旱耐受性

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Sweet cherry is a non-climacteric fruit and its ripening is regulated by abscisic acid (ABA) during fruit development. In this study, four cDNAs (PacCYP707A1-4) encoding 8'-hydroxylase, a key enzyme in the oxidative catabolism of ABA, were identified in sweet cherry fruits using tobacco rattle virus-induced gene silencing (VIGS) and particle bombardment approaches. Quantitative real-time PCR confirmed significant down-regulation of target gene transcripts in VIGS-treated cherry fruits. In PacCYP707A2-RNAi-treated fruits, ripening and fruit colouring were promoted relative to control fruits, and both ABA accumulation and PacNCED1 transcript levels were up-regulated by 140%. Silencing of PacCYP707A2 by VIGS significantly altered the transcripts of both ABA-responsive and ripening-related genes, including the ABA metabolism-associated genes NCED and CYP707A, the anthocyanin synthesis genes PacCHS, PacCHI, PacF3H, PacDFR, PacANS, and PacUFGT, the ethylene biosynthesis gene PacACO1, and the transcription factor PacMYBA. The promoter of PacMYBA responded more strongly to PacCYP707A2-RNAi-treated fruits than to PacCYP707A1-RNAi-treated fruits. By contrast, silencing of PacCYP707A1 stimulated a slight increase in fruit colouring and enhanced resistance to dehydration stress compared with control fruits. These results suggest that PacCYP707A2 is a key regulator of ABA catabolism that functions as a negative regulator of fruit ripening, while PacCYP707A1 regulates ABA content in response to dehydration during fruit development.
机译：甜樱桃是一种非更年生的果实，其成熟在水果发育过程中受脱落酸（ABA）调节。在本研究中，在甜樱桃诱导的基因沉默（Vigs）和粒子轰击方法中，在甜樱桃果实中鉴定了四种CDNA（PACCYP707A1-4）编码8'-羟基化酶的关键酶。定量实时PCR在Vigs处理的樱桃果实中证实了靶基因转录物的显着下调。在PACCYP707A2-RNAI处理的水果中，相对于对照果实促进了成熟和果实着色，并且ABA积聚和PACNCED1转录水平均上调140％。 Vigs的Paccyp707a2的沉默显着改变了ABA响应和熟化相关基因的转录物，包括ABA代谢相关基因Nced和Cyp707a，花青素合成基因Pacchs，Pacchi，Pacf3h，PacDFR，PACAN和PACUFGT，乙烯生物合成基因PACACO1和转录因子PACMYBA。 PACMYBA的启动子对PACCOP707A2-RNAI处理的水果更强烈地反应，而不是PACCOP707A1-RNAI处理的水果。相比之下，与对照水果相比，PACCYP707A1的沉默刺激果实着色和增强的脱水应力的抗性耐高采烈。这些结果表明，PACCYP707A2是ABA分解代谢的关键调节因子，其用作果实成熟的负调节器，而PACCYP707A1在水果发育过程中响应脱水调节ABA含量。

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