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首页> 外文期刊>Journal of Experimental Botany >The asparagine-rich protein NRP interacts with the Verticillium effector PevD1 and regulates the subcellular localization of cryptochrome 2
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The asparagine-rich protein NRP interacts with the Verticillium effector PevD1 and regulates the subcellular localization of cryptochrome 2

机译：富含天冬酰胺的蛋白质NRP与verticillium效应器Pevd1相互作用，并调节隐色的亚细胞定位2

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摘要

The soil-borne fungal pathogen Verticillium dahliae infects a wide range of dicotyledonous plants including cotton, tobacco, and Arabidopsis. Among the effector proteins secreted by V. dahliae, the 16 kDa PevD1 induces a hypersensitive response in tobacco. Here we report the high-resolution structure of PevD1 with folds resembling a C2 domain-like structure with a calcium ion bound to the C-terminal acidic pocket. A yeast two-hybrid screen, designed to probe for molecular functions of PevD1, identified Arabidopsis asparagine-rich protein (NRP) as the interacting partner of PevD1. Extending the pathway of V. dahliae effects, which include induction of early flowering in cotton and Arabidopsis, NRP was found to interact with cryptochrome 2 (CRY2), leading to increased cytoplasmic accumulation of CRY2 in a blue light-independent manner. Further physiological and genetic evidence suggests that PevD1 indirectly activates CRY2 by antagonizing NRP functions. The promotion of CRY2-mediated flowering by a fungal effector outlines a novel pathway by which an external stimulus is recognized and transferred in changing a developmental program.
机译：土壤传播的真菌病原体verticillium dahliae感染了各种分枝叶植物，包括棉花，烟草和拟南芥。在由V.Dahliae分泌的效应蛋白中，16kDa pevd1在烟草中诱导过敏反应。在这里，我们报告了P​​EVD1的高分辨率结构，其具有类似于C2畴样结构的褶皱，其具有与C末端酸性袋结合的钙离子。酵母双杂交筛，设计用于探测PEVD1的分子功能，确定了富含PEVD1的相互作用伴侣的拟南芥富含乙酰胺的蛋白（NRP）。延长V. Dahliae效应的途径，包括诱导棉花和拟南芥的早期开花，NRP被发现与密集色素2（Cry2）相互作用，导致Cry2的细胞质积累以蓝光独立的方式增加。进一步的生理和遗传证据表明PEVD1间接通过拮抗NRP函数来激活Cry2。通过真菌效应器促进Cry2介导的开花概述了一种新的途径，在改变发展方案时，外部刺激被认可并转移。

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