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首页> 外文期刊>Journal of Experimental Botany >Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice
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Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice

机译：LHC1的LHCA4损伤，LHCI亚基导致叶绿素的高积累和水稻中的温度绿色表型

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摘要

Chlorophyll is an essential molecule for acquiring light energy during photosynthesis. Mutations that result in chlorophyll retention during leaf senescence are called 'stay-green' mutants. One of the several types of stay-green mutants, Type E, accumulates high levels of chlorophyll in the pre-senescent leaves, resulting in delayed yellowing. We isolated delayed yellowing1-1 (dye1-1), a rice mutant whose yellowing is delayed in the field. dye1-1 accumulated more chlorophyll than the wild-type in the pre-senescent and senescent leaves, but did not retain leaf functionality in the 'senescent green leaves', suggesting that dye1-1 is a Type E stay-green mutant. Positional cloning revealed that DYE1 encodes Lhca4, a subunit of the light-harvesting complex I (LHCI). In dye1-1, amino acid substitution occurs at the location of a highly conserved amino acid residue involved in pigment binding; indeed, a severely impaired structure of the PSI-LHCI super-complex in dye1-1 was observed in a blue native PAGE analysis. Nevertheless, the biomass and carbon assimilation rate of dye1-1 were comparable to those in the wild-type. Interestingly, Lhcb1, a trimeric LHCII protein, was highly accumulated in dye1-1, in the chlorophyll-protein complexes. The high accumulation of LHCII in the LHCI mutant dye1 suggests a novel functional interaction between LHCI and LHCII.
机译：叶绿素是用于在光合作用期间获取光能的必要分子。导致叶片衰老期间叶绿素保留的突变被称为“留温度绿色”突变体。几种类型的温度绿色突变体之一，E型，在衰老叶中累积了高水平的叶绿素，导致延迟变黄。我们被隔离延迟呈黄色1-1（DYE1-1），稻米突变体延迟在该领域。染料1-1累积比衰老和衰老叶中的野生型更加叶绿素，但在“衰老绿叶”中没有保留叶功能，表明染料1-1是E型留型绿色突变体。定位克隆显示Dye1编码LHCA4，光收获络合物I（LHCI）的亚基。在染料1-1中，氨基酸取代发生在涉及颜料结合的高度保守的氨基酸残基的位置;实际上，在蓝色天然页面分析中观察到染料1-1中的PSI-LHCI超复合物的严重受损的结构。然而，染料1-1的生物质和碳同化率与野生型中的生物质和碳同化率相当。有趣的是，LHCB1是三聚体LHCII蛋白在叶绿素 - 蛋白复合物中的染料1-1中高积累。 LHCI突变体染料1中LHCII的高积累表明LHCI和LHCII之间的新功能相互作用。

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