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首页> 外文期刊>Journal of Experimental Botany >The bHLH transcription factor GmPIB1 facilitates resistance to Phytophthora sojae in Glycine max
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The bHLH transcription factor GmPIB1 facilitates resistance to Phytophthora sojae in Glycine max

机译：BHLH转录因子GMPIB1有利于甘氨酸最大的抗植物肺炎SOJAE

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摘要

Phytophthora sojae Kaufmann and Gerdemann causes Phytophthora root rot, a destructive soybean disease worldwide. A basic helix-loop-helix (bHLH) transcription factor is thought to be involved in the response to P. sojae infection in soybean, as revealed by RNA sequencing (RNA-seq). However, the molecular mechanism underlying this response is currently unclear. Here, we explored the function and underlying mechanisms of a bHLH transcription factor in soybean, designated GmPIB1 (P. sojae-inducible bHLH transcription factor), during host responses to P. sojae. GmPIB1 was significantly induced by P. sojae in the resistant soybean cultivar 'L77-1863'. Analysis of transgenic soybean hairy roots with elevated or reduced expression of GmPIB1 demonstrated that GmPIB1 enhances resistance to P. sojae and reduces reactive oxygen species (ROS) accumulation. Quantitative reverse transcription PCR and chromatin immunoprecipitation-quantitative PCR assays revealed that GmPIB1 binds directly to the promoter of GmSPOD1 and represses its expression; this gene encodes a key enzyme in ROS production. Moreover, transgenic soybean hairy roots with GmSPOD1 silencing through RNA interference exhibited improved resistance to P. sojae and reduced ROS generation. These findings suggest that GmPIB1 enhances resistance to P. sojae by repressing the expression of GmSPOD1.
机译：Phytophthora Sojae Kaufmann和Gerdemann会导致植物肺症根腐，全世界具有破坏性的大豆疾病。认为基本的螺旋环 - 螺旋（BHLH）转录因子参与大豆在大豆中对P. Sojae感染的反应，如RNA测序（RNA-SEQ）所揭示的。然而，这种反应的分子机制目前不清楚。在这里，我们探讨了大豆中BHLH转录因子的功能和潜在机制，指定GMPIB1（P. Sojae-Imbible BHLH转录因子），在对P. Sojae的宿主反应期间。 GMPIB1在抗性大豆品种'L77-1863'中被P. Sojae显着诱导。转基因大豆毛毛的分析具有升高或降低的GMPIB1表达表明GMPIB1增强了对P. Sojae的耐药性并减少了反应性氧物种（ROS）积累。定量逆转录PCR和染色质免疫沉淀定量PCR测定显示GMPIB1直接与GMSPOD1的启动子结合并抑制其表达;该基因编码ROS生产中的关键酶。此外，具有通过RNA干扰的GMSPOD1沉默的转基因大豆毛状根表现出对P. Sojae的改善性和降低的ROS生成。这些发现表明GMPIB1通过压制GMSPOD1的表达来增强对P. Sojae的抗性。

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  • 来源
    《Journal of Experimental Botany》 |2018年第10期|共15页
  • 作者

    Cheng Qun; Dong Lidong; Gao Tianjiao; Liu Tengfei; Li Ninghui; Wang Le; Chang Xin; Wu Junjiang; Xu Pengfei; Zhang Shuzhen;

  • 作者单位

    Northeast Agr Univ Chinese Educ Minist Key Lab Soybean Biol Soybean Res Inst Harbin 150030 Heilongjiang Peoples R China;

    Northeast Agr Univ Chinese Educ Minist Key Lab Soybean Biol Soybean Res Inst Harbin 150030 Heilongjiang Peoples R China;

    Northeast Agr Univ Chinese Educ Minist Key Lab Soybean Biol Soybean Res Inst Harbin 150030 Heilongjiang Peoples R China;

    Northeast Agr Univ Chinese Educ Minist Key Lab Soybean Biol Soybean Res Inst Harbin 150030 Heilongjiang Peoples R China;

    Northeast Agr Univ Chinese Educ Minist Key Lab Soybean Biol Soybean Res Inst Harbin 150030 Heilongjiang Peoples R China;

    Northeast Agr Univ Chinese Educ Minist Key Lab Soybean Biol Soybean Res Inst Harbin 150030 Heilongjiang Peoples R China;

    Northeast Agr Univ Chinese Educ Minist Key Lab Soybean Biol Soybean Res Inst Harbin 150030 Heilongjiang Peoples R China;

    Heilongjiang Acad Agr Sci Key Lab Soybean Cultivat Minist Agr PR China Soybean Res Inst Harbin 150086 Heilongjiang Peoples R China;

    Northeast Agr Univ Chinese Educ Minist Key Lab Soybean Biol Soybean Res Inst Harbin 150030 Heilongjiang Peoples R China;

    Northeast Agr Univ Chinese Educ Minist Key Lab Soybean Biol Soybean Res Inst Harbin 150030 Heilongjiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    bHLH transcription factor; Glycine max; Phytophthora sojae; root; ROS;

    机译：BHLH转录因子;甘氨酸Max;植物;root;ROS;

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