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首页> 外文期刊>Journal of Experimental Botany >Identification and characterization of terpene synthase genes accounting for volatile terpene emissions in flowers of Freesia x hybrida
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Identification and characterization of terpene synthase genes accounting for volatile terpene emissions in flowers of Freesia x hybrida

机译：萜烯合成酶基因综合征术对小型X Hybrida挥发性萜烯排放的鉴定及表征

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摘要

The development of flower scents was a crucial event in biological evolution, providing olfactory signals by which plants can attract pollinators. In this study, bioinformatics, metabolomics, and biochemical and molecular methodologies were integrated to investigate the candidate genes involved in the biosynthesis of volatile components in two cultivars of Freesia x hybrida, Red River (R) and Ambiance, which release different categories of compounds. We found that terpene synthase (TPS) genes were the pivotal genes determining spatiotemporal release of volatile compounds in both cultivars. Eight FhTPS genes were isolated and six were found to be functional: FhTPS1 was a single-product enzyme catalyzing the formation of linalool, whereas the other four FhTPS proteins were multi-product enzymes, among which FhTPS4, FhTPS6, and FhTPS7 could recognize geranyl diphosphate and farnesyl diphosphate simultaneously. The FhTPS enzymatic products closely matched the volatile terpenes emitted from flowers, and significant correlations were found between release of volatile terpenes and FhTPS gene expression. Graphical models based on these results are proposed that summarize the biosynthesis of Freesia floral volatile terpenes. The characterization of FhTPS genes paves the way to decipher their roles in the speciation and fitness of Freesia, and this knowledge could also be used to introduce or enhance scent in other plants.
机译：花卉香味的发展是生物进化中的一个关键事件，提供了植物可以吸引传染案的嗅觉信号。在本研究中，整合生物信息学，代谢组科和生化和分子方法，以研究涉及两种美味X Hybrida，Red River（R）和氛围中的挥发性组分生物合成的候选基因，释放不同类别的化合物。我们发现萜烯合酶（TPS）基因是测定两种品种中挥发性化合物的挥发性释放的枢轴基因。分离出8个FHTPS基因，发现六种功能性：FHTPS1是催化丙纳的形成的单一产品酶，而其他四种FHTPS蛋白是多种产品，其中FHTPS4，FHTPS6和FHTPS7可以识别二烷基二磷酸酯和法呢基二磷酸同时。 FHTPS酶促产品与鲜花发射的挥发性Terpenes紧密相匹配，并且在挥发性Terpenes和FHTPS基因表达的释放之间发现了显着的相关性。提出了基于这些结果的图形模型，总结了Freesia花卉挥发性Terpenes的生物合成。 FHTPS基因的表征铺平了破译的方式，在弗雷群岛的形态和适应性中解读了它们的作用，并且这些知识也可用于在其他植物中引入或增强气味。

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