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首页> 外文期刊>Journal of Experimental Botany >Gnp4/LAX2, a RAWUL protein, interferes with the OsIAA3-OsARF25 interaction to regulate grain length via the auxin signaling pathway in rice
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Gnp4/LAX2, a RAWUL protein, interferes with the OsIAA3-OsARF25 interaction to regulate grain length via the auxin signaling pathway in rice

机译：GNP4 / LAX2，Rawul蛋白，干扰OSIAA3-OSARF25相互作用，通过水稻中的毒素信号通路调节粒度

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摘要

Grain length is one of the determinants of yield in rice and auxin plays an important role in regulating it by mediating cell growth. Although several genes in the auxin pathway are involved in regulating grain length, the underlying molecular mechanisms remain unclear. In this study we identify a RING-finger and wd40-associated ubiquitin-like (RAWUL) domain-containing protein, Gnp4/LAX2, with a hitherto unknown role in regulation of grain length by its influence on cell expansion. Gnp4/LAX2 is broadly expressed in the plant and subcellular localization analysis shows that it encodes a nuclear protein. Overexpression of Gnp4/LAX2 can significantly increase grain length and thousand-kernel weight. Moreover, Gnp4/LAX2 physically interacts with OsIAA3 and consequently interferes with the OsIAA3-OsARF25 interaction in vitro and in vivo. OsIAA3 RNAi plants consistently exhibit longer grains, while the mutant osarf25 has small grains. In addition, OsARF25 binds to the promoter of OsERF142/SMOS1, a regulator of organ size, and positively regulates its expression. Taken together, the results reveal that Gnp4/LAX2 functions as a regulator of grain length through participation in the OsIAA3-OsARF25-OsERF142 pathway and that it has potential value for molecular breeding in rice.
机译：谷物长度是水稻产率的决定因素之一，植物素在通过介导细胞生长来调节它的重要作用。虽然制冷途径中的几个基因涉及调节粒度，但下面的分子机制仍然不清楚。在这项研究中，我们鉴定了含有戒指和WD40相关的泛素样（Rawul）含有结构域的蛋白质GNP4 / LAX2，其通过其对细胞膨胀的影响，在粒度的调节中具有迄今未知的作用。 GNP4 / LAX2在植物和亚细胞定位分析中广泛表达，表明它编码了核蛋白质。 GNP4 / LAX2的过度表达可以显着增加粒度长度和千核重量。此外，GNP4 / LAX2物理地与OSIAA3相互作用，因此干扰OSIAA3-OSARF25在体外和体内的相互作用。 Osiaa3 rnai植物一直表现出更长的谷物，而突变体osarf25具有小颗粒。此外，osarf25与OSERF142 / SMOS1的启动子结合，器官尺寸调节剂，并积极调节其表达。占据，结果表明，GNP4 / LAX2通过参与OSIAA3-OSARF25-OSERF142途径作为晶粒长度的调节剂，并且它具有水稻中分子育种的潜在价值。

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  • 来源
    《Journal of Experimental Botany》 |2018年第20期|共15页
  • 作者

    Zhang Zhanying; Li Jinjie; Tang Zuoshun; Sun Xingming; Zhang Hongliang; Yu Jianping; Yao Guoxin; Li Gangling; Guo Haifeng; Li Jilong; Wu Huamao; Huang Hanguang; Xu Yawen; Yin Zhigang; Qi Yanhua; Huang Rongfeng; Yang Weicai; Li Zichao;

  • 作者单位

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    Chinese Acad Sci Inst Genet &

    Dev Biol State Key Lab Mol &

    Dev Biol Beijing 100101 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    Chinese Acad Sci Inst Genet &

    Dev Biol State Key Lab Mol &

    Dev Biol Beijing 100101 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

    Zhejiang Univ Coll Life Sci State Key Lab Plant Physiol &

    Biochem Hangzhou 310058 Zhejiang Peoples R China;

    Chinese Acad Agr Sci Biotechnol Res Inst Beijing 100081 Peoples R China;

    Chinese Acad Sci Inst Genet &

    Dev Biol State Key Lab Mol &

    Dev Biol Beijing 100101 Peoples R China;

    China Agr Univ Coll Agron &

    Biotechnol Key Lab Crop Genet Improvement Key Lab Crop Heterosis &

    Utilizat Minist Educ Beijing 100193 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    Auxin; Gnp4/LAX2; grain length; rice; transcription regulation;

    机译：植物素;GNP4 / LAX2;粒度;米;转录规则;

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