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首页> 外文期刊>Journal of Experimental Botany >Arabidopsis cyclic nucleotide-gated channel 6 is negatively modulated by multiple calmodulin isoforms during heat shock
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Arabidopsis cyclic nucleotide-gated channel 6 is negatively modulated by multiple calmodulin isoforms during heat shock

机译：拟南芥循环核苷酸门控通道6通过热休克期间的多个钙调蛋白同种型对均衡调节

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摘要

An increased concentration of cytosolic Ca2+ is an early response of plant cells to heat shock. Arabidopsis cyclic nucleotide-gated ion channel 6 (CNGC6) mediates heat-induced Ca2+ influx and is activated by cAMP. However, it remains unclear how the Ca2+ conductivity of CNGC6 is negatively regulated under the elevated cytosolic Ca2+ concentration. In this study, Arabidopsis calmodulin isoforms CaM1/4, CaM2/3/5, CaM6, and CaM7 were found to bind to CNGC6 to varying degrees, and this binding was dependent on the presence of Ca2+ and IQ(6), an atypical isoleucine-glutamine motif in CNGC6. Knockout of CaM2, CaM3, CaM5, and CaM7 genes led to a marked increase in plasma membrane inward Ca2+ current under heat shock conditions; however, knockout of CaM1, CaM4, and CaM6 genes had no significant effect on plasma membrane Ca2+ current. Moreover, the deletion of 10 6 from CNGC6 led to a marked increase in plasma membrane Ca2+ current under heat shock conditions. Taken together, the data suggest that CNGC6-mediated Ca2+ influx is likely to be negatively regulated by CaM2/3/5 and CaM7 isoforms under heat shock conditions, and that IQ(6) plays an important role in CaM binding and the feedback regulation of the channel.
机译：胞质CA2 +的浓度增加是植物细胞对热休克的早期反应。拟南芥循环核苷酸门通道6（CNGC6）介导热诱导的Ca2 +流入，并由阵营激活。然而，仍不清楚CNGC6的CA2 +电导率在升高的细胞溶质CA2 +浓度下对CN2 +电导率产生负面调节。在该研究中，发现拟南芥钙调蛋白同种型CAM1 / 4，CAM2 / 3/5，CAM6和CAM7与CNGC6结合到不同程度，并且该结合依赖于CA2 +和IQ（6）的存在，一种非典型异亮氨酸-glutamine主题在CNGC6中。 CAM2，CAM3，CAM5和CAM7基因的敲除导致热冲击条件下的质膜膜中的质量增加。然而，CAM1，CAM4和CAM6基因的敲除对血浆膜Ca2 +电流没有显着影响。此外，在CNGC6中缺失10 6导致热休克条件下的血浆膜Ca2 +电流的显着增加。占据了数据，表明CNGC6介导的CA2 +流入可能是通过热休克条件下的CAM2 / 3/5和CAM7同种型负调节，并且IQ（6）在CAM结合和反馈调节中起重要作用这个频道。

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  • 来源
    《Journal of Experimental Botany》 |2020年第1期|共15页
  • 作者

    Niu Wei-Tao; Han Xiao-Wei; Wei Shan-Shan; Shang Zhong-Lin; Wang Jing; Yang De-Wei; Fan Xiao; Gao Fei; Zheng Shu-Zhi; Bai Jiao-Teng; Zhang Bo; Wang Zi-Xuan; Li Bing;

  • 作者单位

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

    Hebei Normal Univ Coll Life Sci Hebei Collaborat Innovat Ctr Cell Signaling Minis Hebei Key Lab Mol &

    Cellular Biol Key Lab Mol &

    C Shijiazhuang 050024 Hebei Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    Arabidopsis; Ca2+ conductance; CaM; CaM binding domain; CNGC6; heat shock;

    机译：拟南芥;CA2 +电导;CAM;CAM结合域;CNGC6;热休克;

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