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首页> 外文期刊>Journal of Experimental Botany >OsMADS18, a membrane-bound MADS-box transcription factor, modulates plant architecture and the abscisic acid response in rice
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OsMADS18, a membrane-bound MADS-box transcription factor, modulates plant architecture and the abscisic acid response in rice

机译：Osmads18，膜结合的疯箱转录因子，调制植物建筑和水稻中的脱落酸反应

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摘要

The APETALA1 (AP1)/FRUITFULL (FUL)-like transcription factor OsMADS18 plays diverse functions in rice development, but the underlying molecular mechanisms are far from fully understood. Here, we report that down-regulation of OsMADS18 expression in RNAi lines caused a delay in seed germination and young seedling growth, whereas the overexpression of OsMADS18 produced plants with fewer tillers. In targeted OsMADS18 genome-edited mutants (osmads18-cas9), an increased number of tillers, altered panicle size, and reduced seed setting were observed. The EYFP-OsMADS18 (full-length) protein was localized to the nucleus and plasma membrane but the EYFP-OsMADS18-N (N-terminus) protein mainly localized to the nucleus. The expression of OsMADS18 could be stimulated by abscisic acid (ABA), and ABA stimulation triggered the cleavage of HA-OsMADS18 and the translocation of OsMADS18 from the plasma membrane to the nucleus. The inhibitory effect of ABA on seedling growth was less effective in the OsMADS18-overexpressing plants. The expression of a set of ABA-responsive genes was significantly reduced in the overexpressing plants. The phenotypes of transgenic plants expressing EYFP-OsMADS18-N resembled those observed in the osmads18-cas9 mutants. Analysis of the interaction of OsMADS18 with OsMADS14, OsMADS15, and OsMADS57 strongly suggests an essential role for OsMADS18 in rice development.
机译：Apetala1（AP1）/ Fruitfull（Ful） - 状转录因子Osmads18在水稻开发中起多样的功能，但潜在的分子机制远非完全理解。在这里，我们报告说，在RNAI线中奥姆曼德的表达下调导致种子萌发和幼苗生长的延迟，而OSMADS18的过度表达用较少的分蘖产生植物。在靶向OSMADS18基因组编辑突变体（OSMADS18-CAS9）中，观察到增加的耕克，改变的穗尺寸和降低的种子设定。 EyFP-OSMADS18（全长）蛋白质被定位于核和血浆膜，但EYFP-OSMADS18-N（N-末端）蛋白主要局部局限于细胞核。可以通过脱离酸（ABA）刺激OSMADS18的表达，并且ABA刺激引发了HA-OSMADS18的切割，并从血浆膜到核的奥姆玛18的易位。 ABA对幼苗生长的抑制作用在OSMADS18过表达植物中效果较小。过表达植物中，一组ABA响应基因的表达显着降低。表达EyFP-OSMADS18-N的转基因植物的表型类似于OSMADS18-CAS9突变体中观察到的那些。奥斯曼人18，奥姆马德斯15和奥姆曼德斯57对奥姆曼德斯18的互动分析强烈暗示了奥姆曼人数18在水稻发展中的重要作用。

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  • 来源
    《Journal of Experimental Botany》 |2019年第15期|共15页
  • 作者

    Yin Xiaoming; Liu Xiong; Xu Buxian; Lu Piaoyin; Dong Tian; Yang Di; Ye Tiantian; Feng Yu-Qi; Wu Yan;

  • 作者单位

    Wuhan Univ Coll Life Sci State Key Lab Hybrid Rice Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ Coll Life Sci State Key Lab Hybrid Rice Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ Coll Life Sci State Key Lab Hybrid Rice Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ Coll Life Sci State Key Lab Hybrid Rice Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ Coll Life Sci State Key Lab Hybrid Rice Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ Coll Life Sci State Key Lab Hybrid Rice Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ Dept Chem Key Lab Analyt Chem Biol &

    Med Minist Educ Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ Dept Chem Key Lab Analyt Chem Biol &

    Med Minist Educ Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ Coll Life Sci State Key Lab Hybrid Rice Wuhan 430072 Hubei Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    ABA; MADS-box; membrane-bound transcription factor; plant architecture; rice; tiller;

    机译：ABA;MADS-BOX;膜结合的转录因子;植物建筑;米;耕作;

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