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首页> 外文期刊>AIDS >Plasmacytoid dendritic cells and myeloid cells differently contribute to B-cell-activating factor belonging to the tumor necrosis factor superfamily overexpression during primary HIV infection
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Plasmacytoid dendritic cells and myeloid cells differently contribute to B-cell-activating factor belonging to the tumor necrosis factor superfamily overexpression during primary HIV infection

机译：在原发性HIV感染期间，浆细胞样树突状细胞和髓样细胞对属于肿瘤坏死因子超家族过表达的B细胞活化因子的贡献不同

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Background:After describing heightened levels of circulating B-cell-activating factor belonging to the tumor necrosis factor superfamily (BAFF) as well as changes in B-cell phenotype and functions during acute infection by simian immunodeficiency virus, we wanted to determine whether and by which cells BAFF was over-expressed in primary HIV-infected (PHI) patients.Design and methods:We simultaneously examined circulating BAFF levels by ELISA and membrane-bound BAFF (mBAFF) expression by flow cytometry in peripheral blood mononuclear cells of healthy donors and PHI patients followed for 6 months. We also examined whether HIV-1 modifies BAFF expression or release in various myeloid cells and plasmacytoid dendritic cells (pDC) in vitro.Results:Circulating BAFF levels were transiently increased at enrolment. They positively correlated with CXCL10 levels and inversely with B-cell counts. Whereas mBAFF was expressed by most pDC and on a fraction of intermediate monocytes in healthy donors, the frequency of mBAFF(+) cells significantly increased among nonclassical monocytes and CD1c(+) dendritic cells but decreased among pDC in PHI patients. In contrast to myeloid cells, pDC never released BAFF upon stimulation. Their mBAFF expression was enhanced by HIV-1, independently of type I IFN.Conclusion:Our findings reveal that the pattern of BAFF expression by myeloid cells and pDC is altered in PHI patients and constitutes a valuable marker of immune activation whose circulating levels correlate with CXCL10 levels. Due to their homing in different tissue areas, pDC and myeloid cells might target different B-cell subsets through their mBAFF expression or soluble BAFF release.
机译：背景：描述了猿猴免疫缺陷病毒急性感染过程中属于肿瘤坏死因子超家族（BAFF）的循环B细胞活化因子水平升高以及B细胞表型和功能的变化后，我们想确定是否设计和方法：我们同时通过ELISA检测流血BAFF水平和流式细胞仪检测健康献血者外周血单个核细胞中膜结合BAFF（mBAFF）的表达。 PHI患者随访了6个月。我们还检测了HIV-1是否在体外改变了各种髓样细胞和浆细胞样树突状细胞（pDC）中BAFF的表达或释放。结果：入选时循环中BAFF的水平瞬时升高。它们与CXCL10水平呈正相关，与B细胞计数呈负相关。在健康献血者中，大多数pDC和一部分中间单核细胞表达mBAFF，而PHI患者中，非经典单核细胞和CD1c（+）树突状细胞中mBAFF（+）细胞的频率显着增加，而在pDC中则降低。与骨髓细胞相反，pDC刺激后从未释放BAFF。结论：我们的研究结果表明，PHI患者中髓样细胞和pDC的BAFF表达模式发生了改变，并且它们是免疫激活的重要标志物，其循环水平与HIF相关。 CXCL10级别。由于pDC和骨髓细胞归巢于不同的组织区域，它们可能通过其mBAFF表达或可溶性BAFF释放靶向不同的B细胞亚群。

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