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首页> 外文期刊>Biotechnology Advances: An International Review Journal >Growing E. coli to high cell density--a historical perspective on method development.
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Growing E. coli to high cell density--a historical perspective on method development.

机译：使大肠杆菌生长到高细胞密度-方法开发的历史观点

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摘要

E. coli is the major bacterial platform for expressing simple heterologous proteins. Growing E. coli to high densities has been the subject of numerous studies since the early 1970s, exploring the limits of bacterial culture density in order to achieve maximum productivity. Research strategies were focused on improving the cultivation techniques, manipulating the bacteria's physiology or both. As a result, batch, fed batch and dialysis fermentation techniques had been developed. These growth strategies, together with optimization of media composition and the application of molecular biology methods, made it possible to grow E. coli to cell densities of up to 190 g/l (dry weight), while avoiding media precipitation and preventing acetate accumulation. Additional research on the effects of heterologous protein biosynthesis on signal transduction, proteolysis and post transcription events in E. coli may improve its productivity.
机译：大肠杆菌是表达简单异源蛋白质的主要细菌平台。自1970年代初以来，使大肠杆菌生长到高密度一直是众多研究的主题，探索细菌培养物密度的极限以实现最大生产率。研究策略集中在改善栽培技术，操纵细菌的生理学或两者兼而有之。结果，开发了分批，补料分批和透析发酵技术。这些生长策略，加上培养基组成的优化和分子生物学方法的应用，使大肠杆菌能够生长到高达190 g / l（干重）的细胞密度，同时避免了培养基沉淀和防止乙酸盐积累。有关异源蛋白质生物合成对大肠杆菌中信号转导，蛋白水解和转录后事件影响的其他研究可能会提高其生产率。

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