...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of genetics >Chromosomal localization of rDNA and DAPI bands in solanaceous medicinal plant Hyoscyamus niger L.
【24h】

Chromosomal localization of rDNA and DAPI bands in solanaceous medicinal plant Hyoscyamus niger L.

机译：索甘蓝药用植物Hyoscyamus尼日尔L的RDNA和DAPI带的染色体定位。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The genus Hyoscyamus comprises of medicinally important species rich in tropane alkaloids. Its cultivated species have been subjected to genetic enhancement through chromosomal manipulation. Linear differentiation of chromosomes is quite important to facilitate chromosome identification, and inter-chromosomal and intra-chromosomal alterations for complementing cytogenetic analysis. Therefore, two-colour simultaneous fluorescence in situ hybridization was performed on Hyoscyamus niger (2n = 34) that exhibits two prominent 45S rDNA and one weak 5S rDNA site in the chromosome complement. DAPI (4',6-diamidino-2-phenylindole) banding pattern observed on the same metaphase revealed that 15 of the 17 chromosomes have DAPI-positive bands, of which 13 show DAPI-positive bands in the telomeric region of short arm. The two chromosomes that support secondary constriction had only weak DAPI-positive bands in their centromeric region, but their 45S rDNA chromosomal site showed DAPI-negative bands. Chromosomes 4 and 17 did not show any DAPI band. Taken in conjunction with the karyomorphology, the two 45S rDNA sites could be correlated to one site each in the secondary constriction region of chromosomes 5 and 10, and 5S rDNA site in the intercalary region proximal to secondary constriction region of chromosome 5. The occurrence of reduced DAPI fluorescence in the satellite region of chromosome 5 in the 45S rDNA-rich region seems to be a unique observation with respect to molecular composition of this region in H. niger.
机译：静脉瘤属包括富含稀氧烷生物碱的药用物种。通过染色体操纵，其栽培物种已经受到遗传增强。染色体的线性分化对于促进染色体鉴定以及用于补充细胞遗传学分析的染色体型和染色体 - 染色体染色体改变。因此，对原位杂交的双色同时荧光在鼻蛔虫（2N = 34）上进行，其在染色体补充中表现出两个突出的45s rDNA和一个弱5S rDNA位点。在相同的中期观察到的DAPI（4'，6-二氨基-2-苯基吲哚）带状图案显示，17个染色体中的15个具有DAPI阳性带，其中13在短臂的端粒区域中显示DAPI阳性带。支持二次收缩的两条染色体仅在其焦化区域中仅具有弱DAPI阳性带，但它们的45s rDNA染色体位点显示了DAPI负带。染色体4和17没有显示任何DAPI带。结合Karyomorphology携带，两个45s rdNA位点可以与染色体5和10的次要收缩区域的二次收缩区域中的一个位点相关，并且在染色体的次级收缩区域的次级收缩区域近端的闰区域中的5S rdNA位点。发生在45s的富含45s富rDNA的区域的染色体5的卫星区域中的DAPI荧光似乎是关于H. Niger中该区域的分子组合物的独特观察。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号