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首页> 外文期刊>Journal of genetics >Expression profile of genes coding for carotenoid biosynthetic pathway during ripening and their association with accumulation of lycopene in tomato fruits
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Expression profile of genes coding for carotenoid biosynthetic pathway during ripening and their association with accumulation of lycopene in tomato fruits

机译：类胡萝卜素生物合成途径编码的基因的表达谱及其与番茄水果番茄红素积累的关系

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摘要

Fruit ripening process is associated with change in carotenoid profile and accumulation of lycopene in tomato (Solanum lycopersicum L.). In this study, we quantified the β-carotene and lycopene content at green, breaker and red-ripe stages of fruit ripening in eight tomato genotypes by using high-performance liquid chromatography. Among the genotypes, lycopene content was found highest in Pusa Rohini and lowest in VRT-32-1. To gain further insight into the regulation of lycopene biosynthesis and accumulation during fruit ripening, expression analysis of nine carotenoid pathway-related genes was carried out in the fruits of high lycopene genotype-Pusa Rohini. We found that expression of phytoene synthase and β-carotene hydroxylase-1 was four and thirty-fold higher, respectively, at breaker stage as compared to red-ripe stage of fruit ripening. Changes in the expression level of these genes were associated with a 40% increase in lycopene content at red-ripe stage as compared with breaker stage. Thus, the results from our study suggest the role of specific carotenoid pathway-related genes in accumulation of high lycopene during the fruit ripening processes.
机译：果实成熟过程与番茄（Solanum Lycopersicum L.）的类胡萝卜素型材的变化和番茄红素积累有关。在这项研究中，我们通过使用高效液相色谱法在八个番茄基因型中成熟的绿色，断路器和红成熟阶段的β-胡萝卜素和番茄红素含量。在基因型中，吡戊烯含量在Pusa Rohini中发现最高，并且在VRT-32-1中最低。为了进一步了解果丙烯生物合成和果实成熟过程中的积累的进一步了解，在高番茄红素基因型-Pusa rohini的果实中进行了九类胡萝卜素途径相关基因的表达分析。我们发现，与果实成熟的红成熟阶段相比，植物合酶和β-胡萝卜素羟化酶-1的表达分别为5倍和三十倍。与断路器阶段相比，这些基因表达水平的变化与红成熟阶段的番茄红素含量增加40％。因此，我们的研究结果表明，在果实成熟过程中，特定类胡萝卜素途径相关基因在高番茄红素中的积累中的作用。

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