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首页> 外文期刊>Journal of genetics >The utility of NBS-profiling for characterization of yellow rust resistance in an F-6 durum wheat population
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The utility of NBS-profiling for characterization of yellow rust resistance in an F-6 durum wheat population

机译：NBS-剖面的效用，用于在F-6杜兰麦小麦人群中表征黄色锈蚀

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Seedling and adult plant (field) resistance to yellow rust in the durum wheat (Triticum turgidum ssp. durum) cross Kunduru-1149 x Cham-1 was characterized using a functionally-targeted DNA marker system, NBS-profiling. Chi-squared analysis indicated a four gene model conferring seedling yellow rust resistance against Puccinia striiformis f. sp. tritici isolate WYR85/22 (virulent on Yr2, Yr6, Yr7 and Yr9). Interval mapping located two QTL for yellow rust resistance on the long arm of chromosome 1B, while Kruskal-Wallis single marker regression identified a number of additional marker loci associated with seedling and/or adult plant, field resistance to yellow rust. These results suggested that much of the yellow rust resistance seen in the field may be due to seedling expressed resistance (R) genes. Characterization of the DNA sequence of three NBS marker loci indicated that all showed significant homology to functionally-characterized R-genes and resistance gene analogues (RGAs), with the greatest homology being NBS-LRR-type R-genes and RGAs from cereal species.
机译：幼苗和成年植物（田地）耐黄锈病（Triticum Turgidum SSP）的抗性奇平分析表明了促进淘黄锈病的四种基因模型对普兴氏菌毒素F. SP。 Tritici分离wyr85 / 22（毒力在YR2，YR6，YR7和YR9）上。间隔映射位于染色体1B长臂上的黄色锈蚀的两个QTL，而Kruskal-Wallis单标记回归鉴定了与幼苗和/或成人植物相关的许多附加标记基因座，对黄色锈蚀。这些结果表明，该领域中所见的大部分黄色耐锈性可能是由于幼苗表达抗性（R）基因。三个NBS标记锁定的DNA序列的表征表明，所有对功能表征的R-基因和抗性基因类似物（RGA）显示出显着的同源性，具有最大的同源性是来自谷物物种的NBS-LRR型R-Gen和RGA。

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