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首页> 外文期刊>Journal of forensic sciences. >Comparing Standard and Selective Degradation DNA Extraction Methods: Results from a Field Experiment with Sexual Assault Kits
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Comparing Standard and Selective Degradation DNA Extraction Methods: Results from a Field Experiment with Sexual Assault Kits

机译：比较标准和选择性降解DNA提取方法：野外实验与性突击套件的结果

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摘要

A growing number of U.S. cities have large numbers of untested sexual assault kits (SAKs) in police property facilities. Testing older kits and maintaining current case work will be challenging for forensic laboratories, creating a need for more efficient testing methods. Methods: We evaluated selective degradation methods for DNA extraction using actual case work from a sample of previously unsubmitted SAKs in Detroit, Michigan. We randomly assigned 350 kits to either standard or selective degradation testing methods and then compared DNA testing rates and CODIS entry rates between the two groups. Results and conclusions: Continuation- ratio modeling showed no significant differences, indicating that the selective degradation method had no decrement in performance relative to customary methods. Follow-up equivalence tests indicated that CODIS entry rates for the two methods could differ by more than +/- 5%. Selective degradation methods required less personnel time for testing and scientific review than standard testing.
机译：越来越多的美国城市在警察物业设施中拥有大量未经测试的性突击套件（SAKS）。测试较旧的套件和维护当前的案例工作将具有挑战性的法医实验室，创造了更有效的测试方法。方法：使用实际情况从密歇根州底特律的先前未订婚的SAK样本评估DNA提取的选择性降解方法。我们随机分配了350个套件，以标准或选择性降解测试方法，然后比较了两组之间的DNA测试率和Codis进入速率。结果和结论：持续比模型显示出没有显着差异，表明选择性降解方法在相对于常规方法的性能下没有减少。后续等效测试表明，这两种方法的Codis入口率可能超过+/- 5％。选择性降解方法需要较少的人员时间来测试和科学评论而不是标准测试。

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