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首页> 外文期刊>Journal of Ichthyology >Sequencing and Characterisation of Complete Mitogenome DNA for Rasbora hobelmani (Cyprinidae) with Phylogenetic Consideration
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Sequencing and Characterisation of Complete Mitogenome DNA for Rasbora hobelmani (Cyprinidae) with Phylogenetic Consideration

机译：rasbora hobelmani（cyprinidae）与系统发育考虑的完全促滤布组DNA的测序与表征

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摘要

The Kottelat rasbora Rasbora hobelmani is a small ray-finned fish categorized under the genus Rasbora in the Cyprinidae family. In this study, the complete mitogenome sequence of R. hobelmani was sequenced using two pairs of primers covering overlapping regions. The mitogenome is 16 541 bp in length, housing 22 transfer RNA genes, 13 protein-coding genes, two ribosomal RNA genes and one putative control region. Identical gene organisation was spotted between this species and other Rasbora genus members. The heavy strand contains 28 genes while the light strand contains the remaining nine genes. Most protein-coding genes employ ATG as start codon, except for the COI gene, which exploits GTG instead. The central conserved sequence blocks (CSB-F, CSB-E and CSB-D), variable sequence blocks (CSB-3, CSB-2 and CSB-1) as well as the terminal associated sequence (TAS) are conserved in the control region. The maximum likelihood phylogenetic tree revealed the close phylogeny of R. hobelmani with R. sumatrana, R. aprotaenia, R. lateristriata and R. steineri with bootstrap value of at least 99%. This work acts as milestone towards future evolution and population genetics studies of this species as well as the Rasbora genus.
机译：Kottelat Rasbora Rasbora Hobelmani是一个小型射线鱼类，在塞浦路迪族家族中罗斯堡属。在这项研究中，使用覆盖重叠区域的两对引物对R.Hobelmani的完整促滤布组序列进行测序。促滤皮组是16 541bp的长度，壳体22转移RNA基因，13个蛋白质编码基因，两种核糖体RNA基因和一个推定对照区域。在该物种和其他Rasbora属的成员之间发现了相同的基因组织。重型链含有28个基因，而光链含有剩余的九个基因。除CoI基因外，大多数蛋白质编码基因雇用ATG作为起始密码子，其利用GTG。中央保守序列块（CSB-F，CSB-E和CSB-D），可变序列块（CSB-3，CSB-2和CSB-1）以及终端相关序列（TA）在控制中被保守地区。最大似然性系统发育树揭示了R. Hobelmani与R. Sumatrana，R. Aprotaenia，R.Prantistriata和R. Steineri的紧密文学系统。举行价值至少为99％。这项工作充当里程碑，向未来的进化和群体遗传学研究以及rasbora属。

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