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首页> 外文期刊>Journal of industrial microbiology & biotechnology >High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus
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High-affinity transport, cyanide-resistant respiration, and ethanol production under aerobiosis underlying efficient high glycerol consumption by Wickerhamomyces anomalus

机译:高亲和力的运输,抗氰化物呼吸和乙醇产量在有效期下潜在的高效高甘油消耗柳瓜莫马鲁斯

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摘要

Wickerhamomyces anomalus strain LBCM1105 was originally isolated from the wort of cachaca (the Brazilian fermented sugarcane juice-derived Brazilian spirit) and has been shown to grow exceptionally well at high amounts of glycerol. This paramount residue from the biodiesel industry is a promising cheap carbon source for yeast biotechnology. The assessment of the physiological traits underlying the W. anomalus glycerol consumption ability in opposition to Saccharomyces cerevisiae is presented. A new WaStl1 concentrative glycerol-H+ symporter with twice the affinity of S. cerevisiae was identified. As in this yeast, WaSTL1 is repressed by glucose and derepressed/induced by glycerol but much more highly expressed. Moreover, LBCM1105 aerobically growing on glycerol was found to produce ethanol, providing a redox escape to compensate the redox imbalance at the level of cyanide-resistant respiration (CRR) and glycerol 3P shuttle. This work is critical for understanding the utilization of glycerol by non-Saccharomyces yeasts being indispensable to consider their industrial application feeding on biodiesel residue.
机译:Wickerhamomyces Anomalus菌株LBCM1105最初从葡萄蛤(巴西发酵甘蔗汁衍生的巴西精神)中分离出来,并且已被证明在大量甘油中出现非常良好。来自生物柴油行业的这首圆场残留是酵母生物技术的有希望的廉价碳源。提出了对W.Anomalus甘油消费能力依赖于酿酒酵母的生理特性的评估。鉴定了一种新的WASTL1集中性甘油-H + Symporter,其具有两倍于S.酿酒酵母的亲和力。如在该酵母中,通过葡萄糖抑制Wastl1并通过甘油解除抑制/诱导,但更高度表达。此外,发现甘油在甘油上的LBCM1105产生乙醇,提供氧化还原逃逸,以补偿抗氰化物呼吸(CRR)和甘油3P梭水平的氧化还原不平衡。这项工作对于了解非酵母菌酵母的利用至关重要,以考虑对生物柴油残留物的工业应用饲料不可或缺。

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