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首页> 外文期刊>Journal of industrial microbiology & biotechnology >Increase ethyl acetate production in Saccharomyces cerevisiae by genetic engineering of ethyl acetate metabolic pathway
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Increase ethyl acetate production in Saccharomyces cerevisiae by genetic engineering of ethyl acetate metabolic pathway

机译：通过乙酸乙酯代谢途径的基因工程，增加糖粉酿酒酵母中的乙酸乙酯生产

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摘要

Ethyl acetate has attracted much attention as an important chemical raw material and a flavor component of alcoholic beverages. In this study, the biosynthetic pathway for the production of ethyl acetate in Chinese liquor yeast was unblocked. In addition to engineering Saccharomyces cerevisiae to increased intracellular CoA and acetyl-CoA levels, we also increased the combining efficiency of acetyl-CoA to ethanol. The genes encoding phosphopantothenate-cysteine ligase, acetyl-CoA synthetase, and alcohol acetyltransferase were overexpressed by inserting the strong promoter PGK1p and the terminator PGK1t, respectively, and then combine them. Our results finally showed that the ethyl acetate levels of all engineering strains were improved. The final engineering strain CLy12a-ATF1-ACS2-CAB2 had a significant increase in ethyl acetate yield, reaching 610.26 (+/- 14.28)mg/L, and the yield of higher alcohols was significantly decreased. It is proved that the modification of ethyl acetate metabolic pathway is extremely important for the production of ethyl acetate from Saccharomyces cerevisiae.
机译：乙酸乙酯吸引了许多关注作为一种重要的化学原料和酒精饮料的风味组分。在这项研究中，未封闭中国酵母中乙酸乙酯的生物合成途径。除了工程酿酒酵母酿酒酵母外，还增加了细胞内COA和乙酰-COA水平外，我们还增加了乙酰-COA至乙醇的结合效率。通过分别插入强启动子PGK1P和终止子PGK1T，对编码磷酸盐 - 半胱氨酸 - 半胱氨酸 - 半胱氨酸酶，乙酰基-COA合成酶和醇乙酰转移酶的基因进行过表达，然后将它们组合。我们的结果最终表明，所有工程株的乙酸乙酯水平都得到了改善。最终的工程菌株CLY12A-ATF1-ACS2-CAB2具有显着增加的乙酸乙酸乙酯产率，达到610.26（+/- 14.28）Mg / L，较高醇的产率显着降低。事实证明，乙酸乙酯代谢途径的改性对于从酿酒酵母中生产乙酸乙酯是非常重要的。

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