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首页> 外文期刊>Biotechnology Progress >Periodically Discontinuous Induction of Bone Marrow Stem Cells toward Osteogenic Differentiation in Vitro
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Periodically Discontinuous Induction of Bone Marrow Stem Cells toward Osteogenic Differentiation in Vitro

机译：周期性间断诱导骨髓干细胞向成骨细胞分化。

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This paper reveals that a discontinuous in vitro induction, namely, the periodic presence and absence of foreign induction factors, might be, under a certain condition, more effective to stimulate stem cells' differentiation than a continuous induction. Bone marrow stem cells (BMSCs) derived from Sprague Dawley rats were employed to examine the effects of discontinuous additions of osteogenic supplements with a series of alternate frequency in contrast to those with continuous induction or no induction. The results demonstrated that a suitable discontinuous induction was more able to achieve osteogenesis than not only no induction but also the associated continuous induction. Additionally, the osteogenic supplements were confirmed to enhance cell differentiation but suppress cell proliferation. So, the combination of differentiation extent per cell and cell number accounts for the "unexpected" good osteogenic effect of the discontinuous induction. The induction effect was found to be dependent upon alternate frequency, and the optimum alternate period in our experimental systems was determined to be around 4 days. Since it is very common to change culture medium every 2—4 days, such a strategy of discontinuous induction does not bring any extra manual work but reduces the consumption of foreign induction factors and significantly enhances the global differentiation efficacy. Our work thus affords a convenient and practical approach to achieve differentiation of BMSCs, which might be useful for potential large-scale culture and differentiation of stem cells. Meanwhile, the existence of optimum frequency implies some unknown inherent rhythms of cell proliferation and differentiation.
机译：本文揭示了在一定条件下，不连续的体外诱导，即周期性存在和不存在外源诱导因子，可能比连续诱导更有效地刺激干细胞的分化。与连续诱导或不诱导相比，采用源自Sprague Dawley大鼠的骨髓干细胞（BMSC）来检查以一系列交替频率不连续添加成骨补品的效果。结果表明，合适的不连续诱导比不诱导和连续诱导更能实现成骨作用。此外，已确认成骨补充剂可增强细胞分化但抑制细胞增殖。因此，每细胞分化程度和细胞数量的组合构成了不连续诱导的“意外”良好的成骨作用。发现诱导效果取决于交替频率，并且在我们的实验系统中确定最佳交替周期为约4天。由于每2-4天更换一次培养基非常普遍，因此这种不连续诱导的策略不会带来任何额外的手工工作，而是减少了外源诱导因子的消耗并显着提高了整体分化功效。因此，我们的工作提供了一种方便实用的方法来实现BMSCs的分化，这对于潜在的大规模培养和干细胞分化可能是有用的。同时，最佳频率的存在暗示了细胞增殖和分化的一些未知的固有节律。

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