...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of insect biotechnology and sericology >Role of the Duplicate Fructose-1,6-Diphosphate Aldolase Genes in the Silkworm, Bombyx Mori (Lepidoptera): Comparison with a Single Gene in Drosophila Melanogaster (Diptera)
【24h】

Role of the Duplicate Fructose-1,6-Diphosphate Aldolase Genes in the Silkworm, Bombyx Mori (Lepidoptera): Comparison with a Single Gene in Drosophila Melanogaster (Diptera)

机译：重复的果糖-1,6-二磷酸醛糖酶基因在家蚕，Bombyx Mori（鳞翅目）中的作用：与果蝇（Diptera）的单个基因的比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fructose 1,6-bisphosphate aldolase (EC 4.1.2.13.; ALD) is a key enzyme in cellular carbon-carbon bond formation or breakage, and plays roles in both glycolysis and gluconeogenesis in the cytoplasm of higher organisms. Here we report the identification, expression analysis and functional characterization of ALD genes from the silkworm, Bombyx mori. In contrast to the single gene in Drosophila melanogaster, the lepidopteran silkworm genome was found to possess two ALD genes, BmALDI and BmALD2. The phylogenetic tree of the arthropod ALD clearly showed that insect ALDs can be classified into two classes, the typical ALDs present in various insect species (group I; including BmALD1) and the lepidopteran-specific ALDs (group II; including BmALD2). Among the eight larval organs examined, BmALD1 mRNA showed the highest expression in muscle, followed by the head, and BmALD2 was widely expressed except in the head and mid-gut; the highest expression was observed in muscle. The homo-tetramer enzymes of BmALD1and BmALD2 were expressed as recombinant proteins in Escherichia coli (rBmALD1 and rBmALD2, respectively), from which they were purified to homogeneity. Two types of ALD could be distinguished by the activity ratio towards the two substrates fructose 1,6-bisphos-phate (FBP)/fructose 1-phosphate (F1P) and the effect of pH on the activities, and these properties of rBmALD1 resembled those of Drosophila ALD. The results of this study show the indispensable role of B. mori ALDs in the control of tissue-specific sugar-phosphate metabolisms, and will provide a basis for studying the evolution of insect ALD genes.
机译：果糖1,6-双磷酸醛糖酶（EC 4.1.2.13。; ALD）是细胞碳 - 碳键形成或破裂中的关键酶，并在高生物的细胞质中糖酵解和葡糖生成中起作用。在这里，我们报告了来自家蚕，Bombyx Mori的ALD基因的鉴定，表达分析和功能表征。与果蝇中的单个基因相比，发现鳞翅目蚕基因组具有两个ALD基因，BMALDI和BMALD2。节肢动物ALD的系统发育树清楚地表明，昆虫ALD可以分为两类，典型的ALD存在于各种昆虫物种（Ⅰ组;包括BMALD1）和鳞翅目丹特异性ALD（II组;包括BMALD2）。在检查的八个幼虫器官中，BMALD1 mRNA显示肌肉中最高的表达，其次是头部，除头部和中肠中外，BMALD2被广泛表达;在肌肉中观察到最高表达。 BMALD1和BMALD2的同种 - 四聚物酶在大肠杆菌（分别为RBMALD1和RBMALD2）中表达为重组蛋白，将它们纯化为均匀性。通过朝向两个基质果糖1,6-双噬菌体（FBP）/果糖1-磷酸（F1P）的活性比可以区分两种类型的ALD和pH对活性的影响，并且RBMALD1的这些性质类似于那些果蝇ALD。该研究的结果表明B. Mori Alds在控制组织特异性糖 - 磷酸盐代谢中的不可或缺的作用，并将为研究昆虫ALD基因的演变提供依据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号