Detection of cry-type gene homologues in the draft genome sequence of Paenibacillus popilliae ATCC 14706T and trial for the expression of recombinantproteins in Escherichia coli

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Detection of cry-type gene homologues in the draft genome sequence of Paenibacillus popilliae ATCC 14706T and trial for the expression of recombinantproteins in Escherichia coli

机译：检测Paenibacillus Popilliae ATCC 14706T基因组序列草案中的哭型基因同源物及其在大肠杆菌中表达重组蛋白质的试验

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Two novel cry-type gene homologues, tentatively named cryPpI and cryPp2, were detected in the genomic DNA of Paenibacillus popilliae ATCC 14706T, both of which were similar to genes encoding Cry18 proteins, suggesting their similar modes of action. Ours is the first report concerning multiple Cry homologues, detected from a sole strain of P. popilliae. In order to characterize the roles of the two Cry homologues in pathogenicity, a trial for the expression of recombinant proteins in Escherichia coliwas conducted, which revealed that the optimization of rare codons, such as supplementation of tRNA genes, was necessary.

机译：在Paenibacillus Popilliae ATCC 14706T的基因组DNA中检测到两种新的哭型基因同源物，暂时命名的CRYPPI和CRYPP2，两者都与编码CRY18蛋白质的基因类似，表明它们类似的作用方式。我们是从P. Popilliae的唯一菌株检测到多个哭同源物的第一份报告。为了表征两种乳腺同源物中致病性的作用，在进行的大肠杆菌中表达重组蛋白的试验表明，需要稀有密码子的优化，例如补充TRNA基因。

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《Journal of insect biotechnology and sericology》 |2020年第2期|共6页
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正文语种 eng
中图分类蚕桑;
关键词
cry gene; milky disease; Paenibacillus popilliae; protein expression;

机译：哭泣基因;乳白色疾病;Paenibacillus popilliae;蛋白质表达;

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1. Detection of cry-type gene homologues in the draft genome sequence of Paenibacillus popilliae ATCC 14706T and trial for the expression of recombinantproteins in Escherichia coli [J] . Journal of insect biotechnology and sericology . 2020,第2期

机译：检测Paenibacillus Popilliae ATCC 14706T基因组序列草案中的哭型基因同源物及其在大肠杆菌中表达重组蛋白质的试验
2. Draft Genome Sequence of Paenibacillus popilliae ATCC 14706T [J] . Kazuhiro liyama, Kazuki Mor, Hiroaki Mon, Journal of insect biotechnology and sericology . 2013,第2期

机译：芽孢杆菌ATCC 14706的基因组序列草案
3. Draft Genome Sequence of Paenibacillus popilliae ATCC 14706T [J] . Kazuhiro Iiyama, Kazuki Mori, Hiroaki Mon, Journal of insect biotechnology and sericology . 2013,第2期

机译：芽孢杆菌ATCC 14706的基因组序列草案
4. Expression and characterization of an Acidithiobacillus ferrooxidans ATCC23270 heat shock protein hsp90 (AtHtpG) functionally expressed in Escherichia coli [C] . Jianyu Zhu, Qiuxia Huang, Weifeng Jiao, International biohydrometallurgy symposium . 2011

机译：在大肠杆菌中功能表达的铁氧化酸性硫杆菌ATCC23270热休克蛋白hsp90（AtHtpG）的表达和表征
5. Bioinformatic and Sequence Analysis of Four Resuscitation Promoting Factor (Rpf) Gene Homologues in Mycobacterium avium subsp. paratuberculosis (Mpt), and Expression of the Putative Mpt rpfB in Escherichia coli. [D] . Hedlund, David D. 2010

机译：鸟分枝杆菌亚种中四个复苏促进因子（Rpf）基因同源物的生物信息学和序列分析。副结核病（Mpt）和假定的Mpt rpfB在大肠杆菌中的表达。
6. Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706T [O] . Kazuhiro Iiyama, Hiroaki Mon, Kazuki Mori, 2015

机译：流行性芽孢杆菌ATCC 14706T质粒编码的KfrA蛋白的表征
7. Draft Genome Sequence of Escherichia coli K-12 (ATCC 29425) [O] . Putonti, Catherine, Engelbrecht, Kathleen C., Koenig, David W., 2017

机译：大肠杆菌K-12（ATCC 29425）基因组序列草案

Detection of cry-type gene homologues in the draft genome sequence of Paenibacillus popilliae ATCC 14706T and trial for the expression of recombinantproteins in Escherichia coli

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