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首页> 外文期刊>Journal of industrial and engineering chemistry >Simultaneous delivery of DNA vaccine and hydrophobic adjuvant using reducible polyethylenimine-functionalized graphene oxide for activation of dendritic cells
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Simultaneous delivery of DNA vaccine and hydrophobic adjuvant using reducible polyethylenimine-functionalized graphene oxide for activation of dendritic cells

机译：使用可降解聚乙烯官能化石墨烯氧化物同时递送DNA疫苗和疏水佐剂，用于激活树突细胞

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摘要

The activation and maturation of dendritic cells are critical in immunotherapy, and the potency of DCs is associated with high levels of antigen presentation on major histocompatibility complex (MHC) class I and II and the expression of costimulatory signals. Graphene oxide (GO) and its derivatives have many excellent physicochemical properties, including large and hydrophobic surface available for interacting with hydrophobic or aromatic drugs via is-it stacking forces, flexibility of the chemical modification on the surfaces, and capacity of DC activation. In this study, we designed a simple strategy to achieve the co-delivery of a DNA vaccine and hydrophobic immune adjuvant (R848) and to enhance the adjuvanticity of R848 via the synergistic effect of GO and R848. Thiolated low-molecular-weight polyethylenimine (TPEI1.8) was crosslinked with 4-aminothiophenol-modified GO (TGO) via the formation of disulfide bonds. Thus, TGO with its assembled TPEI1.8 could not only load R848 but also electrostatically interact with the DNA vaccine. Owing to the reducibility of the disulfide bond in the cellular environment, the DNA vaccine could be readily released. This system can significantly enhance the DNA transfection, the expression of the costimulatory signal, and the level of antigen presentation to MHC class I DCs for their activation and maturation. (C) 2019 Published by Elsevier B.V. on behalf of The Korean Society of Industrial and Engineering Chemistry.
机译：树突细胞的活化和成熟在免疫疗法中至关重要，DCS的效力与主要组织相容性复合物（MHC）I类和II的高水平抗原呈递相关，以及共刺激信号的表达。石墨烯氧化物（GO）及其衍生物具有许多优异的物理化学性质，包括可用于通过疏水或芳族药物通过IS-IT堆叠力相互作用的大而疏水性表面，化学改性的表面和DC活化的容量。在这项研究中，我们设计了一种简单的策略，以实现DNA疫苗和疏水性免疫佐剂（R848）的共同递送，并通过GO和R848的协同作用来增强R848的佐性。通过形成二硫键与4-氨噻吩醇改性的去（TPEI1.8）与硫醇化的低分子量聚乙烯亚胺（TPEI1.8）交联。因此，具有其组装的TPEI1.8的TGO不仅可以加载R848，而且还可以与DNA疫苗静电相互作用。由于在细胞环境中的二硫键的再还原性，可以容易地释放DNA疫苗。该系统可以显着提高DNA转染，共刺激信号的表达，以及抗原呈现给MHC I类DC的激活和成熟。 （c）2019年由Elsevier B.V发布。代表朝鲜工程和工程化学学会。

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