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首页> 外文期刊>Biotechnology Progress >Effect of Shear Stress on Anthraquinones Production by Rubia tinctorum Suspension Cultures
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Effect of Shear Stress on Anthraquinones Production by Rubia tinctorum Suspension Cultures

机译：剪应力对茜草悬浮培养物中蒽醌产生的影响

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Suspension cultures of Rubia tinctorum,an anthraquinones(AQs)producer,were grown both in Erlenmeyer flasks at 100 rpm and in a 1.5 L mechanically stirred tank bioreactor operating at 450 rpm.The effect of hydrodynamic stress on cell viability,biomass,and AQs production was evaluated.Cell viability showed a transient decrease in the bioreactor during the first days,returning to the initial values toward the end of the culture time.The biomass obtained in the bioreactor was 29% lower than that attained in the Erlenmeyer flasks.The H2O2 production in the bioreactor(with peaks at 7 and 10 days)was about 15 times higher than that obtained in the flasks.A clear relationship exists between the maximum concentration of H2O2 generated and AQs produced.The AQs content in the bioreactor was 233% higher than that in the Erlenmeyer flasks.The AQs specific productivity in the stirred tank and in the Erlenmeyer flasks was 70.7 and 28.5 mu mol/g FW/day,respectively.This production capability was maintained in the regrowth assays.On the other hand,the negative effects of hydrodynamic stress on viability and biomass concentration observed in the bioreactor culture were reverted in the regrowth cultures.It can be concluded that R.tinctorum suspension cultures are able to grow in stirred tanks at 450 rpm responding to the hydrodynamic stress with higher concentrations of AQs,which suggest the possibility of a technological approach taking advantage of this phenomenon.
机译：蒽醌（AQs）生产者魔芋（Rubia tinctorum）的悬浮培养物分别在锥形烧瓶中以100 rpm的速度和在1.5 L机械搅拌槽生物反应器中以450 rpm的速度进行培养。在第一天，生物反应器的细胞活力出现短暂下降，并在培养时间结束时恢复到初始值。在生物反应器中获得的生物量比在锥形瓶中获得的生物量低29％。生物反应器的生产量（在第7天和第10天达到峰值）比烧瓶中的高约15倍。最大生成的H2O2浓度与产生的AQ之间存在明显的关系。生物反应器中的AQs含量高233％搅拌釜和锥形瓶中的AQs比生产率分别为70.7和28.5μmol/ g FW / day。另一方面，在再生培养物中逆转了水动力胁迫对生物反应器培养物中观察到的活力和生物量浓度的负面影响。可以得出结论，丁香菌悬浮培养物能够在搅拌下生长罐以450 rpm的速度响应较高浓度的AQ时对水动力应力的响应，这表明采用这种方法的技术方法的可能性。

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