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首页> 外文期刊>Journal of industrial and engineering chemistry >Rapid identification and quantification of bioactive metabolites in processed Camellia sinensis samples by UHPLC-ESI-MS/MS and evaluation of their antioxidant activity
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Rapid identification and quantification of bioactive metabolites in processed Camellia sinensis samples by UHPLC-ESI-MS/MS and evaluation of their antioxidant activity

机译：UHPLC-ESI-MS / MS加工山茶花Sinensis样品中生物活性代谢物的快速鉴定和定量，并评估其抗氧化活性

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Tea beverages have been enjoyed globally for the last several decades. The present study focuses on the comprehensive chemical and biological analysis of three processed tea products. A total of sixty-three compounds in processed samples were identified based on their exact masses and fragmentation patterns using LC-ESI-QTOF-MS/MS. Furthermore, quantification of eight analytes including caffeine (1), theophylline (2), (+)-catechin (3), (-)-epicatechin (4), (-)-epicatechin gallate (5), (-)-gallocatechin (6), (-)-epigallocatechin gallate (7) and quercetin-3-D-beta-glucoside (8) in tea samples was performed using LC-ESI-IT-MS/MS. The concentrations of analytes were found in the range of 0.03 mg/g to 75.58 mg/g in all tea samples. The developed method showed excellent accuracy as %bias ranged from 0.2-3.69% and a good precision with %RSD ranged from 0.03 to 5.11. The LOD and LOQ for all the analytes were found to be in the range of 0.16-3.26 ng/mL and 0.44-9.87 ng/mL, respectively. The DPPH scavenging effects of samples were also investigated and all the samples were found to be strong scavengers of DPPH radical showing 69.50 +/- 0.01-78.60 +/- 0.10 %RSA. The established method provides a useful way for understanding the metabolite distribution in processed products of C. sinensis and to develop quality control protocols for these products. (C) 2020 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
机译：过去几十年来，茶饮料已经全球享受。本研究侧重于三种加工茶产品的综合化学和生物学分析。使用LC-ESI-QTOF-MS / MS基于它们的精确质量和碎片模式来鉴定加工样品中总共61个化合物。此外，八种分析物的定量包括咖啡因（1），茶碱（2），（+） - 儿茶素（3），（ - ） - EpicateChin（4），（ - ） - EpicateChin Gallate（5），（ - ） - GallocateChin （6），（ - ） - 使用LC-ESI-IT-MS / MS进行茶样品中的EpigallocateChin Gallate（7）和槲皮素-3-D-Beta-葡糖苷（8）。在所有茶样品中发现分析物的浓度在0.03mg / g至75.58mg / g的范围内。开发方法显示出优异的精度，随着0.2-3.69％的％偏压，％RSD的良好精度范围为0.03至5.11。发现所有分析物的LOD和LOQ分别在0.16-3.26ng / ml和0.44-9.87ng / ml的范围内。还研究了样品的DPPH清除效果，发现所有样品都是强烈的DPPH激进术的强化清除剂，显示69.50 +/- 0.01-78.60 +/- 0.10％RSA。建立的方法提供了理解C. Sinensis的加工产品中的代谢物分布，并为这些产品开发质量控制方案的有用方法。 （c）2020朝鲜工业与工程化学学会。 elsevier b.v出版。保留所有权利。

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