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首页> 外文期刊>Biotechnology Progress >Isolation and Identification of Two Novel SDS-Resistant Secreted Chitinases from Aeromonas schubertii
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Isolation and Identification of Two Novel SDS-Resistant Secreted Chitinases from Aeromonas schubertii

机译：schubertii气单胞菌的两种新型抗SDS分泌型几丁质酶的分离与鉴定

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摘要

Two SDS-resistant endochitinases,designated as ASCHI53 and ASCHI61,were isolated from Aeromonas schubertii in a soil sample from southern Taiwan.MALDI-TOF mass measurement indicates the molecular weights of 53,527 for ASCHI53 and 61,202 for ASCHI61.N-terminal and internal amino acid sequences were obtained,and BLAST analysis of the sequences and MS/MS peptide sequencing showed that they were novel proteins.Degradation of chitin by these two endochitinases gave rise to hexameric chitin oligosaccharide,a compound known to have several potent biomedical functions.ASCHI53 and ASCHI61 retained,respectively,65% and 75%,of their chitinase activity in the presence of 5% SDS and 100% of their activity in the presence of 10% beta-mercaptoethanol.These results demonstrate that they are SDS-resistant endochitinases and probably have a rigid structure.
机译：从台湾南部的土壤样品中分离到两个耐SDS的内切壳素酶，分别称为ASCHI53和ASCHI61.MALDI-TOF质量测量表明ASCHI53的分子量为53527，ASCHI61的分子量为61202.N末端和内部氨基酸获得序列，BLAST分析序列和MS / MS肽序列表明它们是新型蛋白质。这两种内切壳多糖酶降解几丁质产生了六聚体几丁质寡糖，该化合物已知具有多种强大的生物医学功能。ASCHI53和ASCHI61在5％SDS的存在下分别保留了65％和75％的几丁质酶活性，在10％β-巯基乙醇的存在下保留了100％的几丁质酶活性。这些结果表明它们是抗SDS的内切壳多糖酶，可能具有刚性结构。

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