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首页> 外文期刊>Biotechnology Progress >Optimization of Medium Composition for the Production of Antimicrobial Activity by Bacillus Subtilis B38
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Optimization of Medium Composition for the Production of Antimicrobial Activity by Bacillus Subtilis B38

机译：枯草芽孢杆菌B38产生抗菌活性的培养基组成的优化

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An antimicrobial activity produced by Bacillus subtilis B38 was found to be effective against several bacteria, including pathogenic and spoilage microorganisms such as, Listeria monocytogenes, Salmonella enteridis, and clinical isolates of methicillin-resistant Staphylococcus species. Nutrients such as carbon, nitrogen sources, and inorganic salts enhanced the production level of the antibacterial activity by B. subtilis B38. A first screening step showed that lactose, ammonium succinate, and manganese most influenced both cell growth and antibacterial activity production. These three factors varied at two levels in eight experiments using full factorial design. Results indicated that maximum cell growth (OD = 10.2) and maximum production of antibacterial activity (360 AU/mL) were obtained in a modified medium containing 1.5% (w/v) lactose, 0.15% (w/v) ammonium succinate, and 0.3 mg/L manganese. Depending on the indicator strain used, the antibacterial activity was 2-to 4-fold higher in the modified culture medium than in TSB medium under the same conditions. Thin layer chromatography-bioautography assay showed the presence of three active spots with R_f values of 0.47, 0.7, and 0.82 in TSB medium. However, the inhibition zone of two spots (Rf values of 0.7 and 0.82) was slightly larger in the modified medium. Moreover, a large zone of inhibition with an R_f value of 0.3, was observed in this modified medium, instead of the spot having an R_f value of 0.47. These results suggest that the nutrients act as environmental factors, quantitatively and qualitatively affecting the production of antibacterial compounds by B. subtilis B38.
机译：发现枯草芽孢杆菌B38产生的抗菌活性可有效抵抗多种细菌，包括病原性和腐败性微生物，如单核细胞增生李斯特菌，肠炎沙门氏菌和耐甲氧西林葡萄球菌的临床分离株。营养物质（例如碳，氮源和无机盐）提高了枯草芽孢杆菌B38产生抗菌活性的水平。第一步筛选显示乳糖，琥珀酸铵和锰对细胞生长和抗菌活性的产生影响最大。在使用完全因子设计的八个实验中，这三个因子在两个级别上有所不同。结果表明，在含有1.5％（w / v）乳糖，0.15％（w / v）琥珀酸铵和1.5％（w / v）乳糖的改良培养基中可获得最大的细胞生长（OD = 10.2）和最大的抗菌活性（360 AU / mL）。 0.3 mg / L锰。根据使用的指示菌株，在相同条件下，改良培养基中的抗菌活性比TSB培养基高2至4倍。薄层色谱-生物医学分析表明在TSB培养基中存在三个活性点，R_f值分别为0.47、0.7和0.82。但是，在改良培养基中，两个斑点的抑制区（Rf值分别为0.7和0.82）稍大。此外，在该改性培养基中观察到具有R_f值为0.3的大抑制区域，而不是R_f值为0.47的斑点。这些结果表明，营养物是环境因素，在数量和质量上影响枯草芽孢杆菌B38产生抗菌化合物。

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