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首页> 外文期刊>Journal of investigative surgery: The official journal of the Academy of Surgical Research >A Gene-Related Nomogram for Preoperative Prediction of Lymph Node Metastasis in Colorectal Cancer
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A Gene-Related Nomogram for Preoperative Prediction of Lymph Node Metastasis in Colorectal Cancer

机译：与淋巴结转移术前预测结直肠癌中的基因相关的NOM图

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摘要

Purpose: To develop and validate a gene-related nomogram for predicting the risk of lymph node (LN) metastasis preoperatively in patients with colorectal cancer (CRC). Methods: RNA-seq data of 581 CRC and 51 normal cases with clinical features were downloaded from TCGA database. In the evaluation cohort with 381 CRC cases, the LASSO regression was used to reduce dimensionality of gene signatures extracted to build gene score. A gene-related nomogram was performed based on the multivariable logistic regression analysis. The performance of the nomogram was assessed by the discrimination, calibration, and clinical usefulness not only in the evaluation, but also in the validation cohort with 200 CRC cases. Results: A total of 12,590 differentially expressed genes were selected, in which 59 candidates associated with LN metastasis in differentially expressed genes set were screened by LASSO to form the gene score. Based on the analysis of multivariate logistic regression, the gene-related nomogram showed good calibration and discrimination not only in the evaluation cohort (concordance-index 0.93; 95%CI 0.91-0.96), but also in the validation cohort (concordance-index 0.70; 95%CI 0.63-0.78). The decision curve analysis of the gene-related nomogram also provides constructive guidance for the design of operation plan, preoperatively. Conclusions: The presented genes nomogram may predict the LN metastasis in CRC patients, preoperatively. And 59 hub genes were defined related to LN metastasis of CRC, which can serve as treatment targets for the further study. Preoperative biopsy and gene analysis are needed to develop the operation plan in clinical practice.
机译：目的：开发和验证与结直肠癌（CRC）患者术前预测淋巴结（LN）转移的风险的基因相关的NOMA图。方法：从TCGA数据库下载581 CRC的RNA-SEQ数据和51个临床特征的正常情况。在评估队列中具有381个CRC病例的评估队列中，套索回归用于减少提取的基因签名的维度，以构建基因评分。基于多变量逻辑回归分析进行了基因相关的NOM图。不仅在评估中的歧视，校准和临床用途评估了NOM图的性能，而且还评估了200个CRC病例的验证队列。结果：选择总计12,590个差异表达基因，其中通过洛索筛选与差异表达基因中的LN转移相关的59个候选物，以形成基因评分。基于对多变量逻辑回归的分析，基因相关的NOM图显示不仅在评估队列中的校准和歧视（一致性索引0.93; 95％CI 0.91-0.96），还显示出良好的校准和歧视，而且还在验证队列中（协调索引0.70 ; 95％CI 0.63-0.78）。基因相关墨迹图的决策曲线分析还为术前，为操作计划设计提供了建设性指导。结论：所呈现的基因载体可以预测术前预测CRC患者的LN转移。和59个枢纽基因与CRC的LN转移相关，其可以作为进一步研究的治疗靶标。需要术前活组织检查和基因分析来开发临床实践中的运作计划。

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    Wei Shuxun; Zang Jia; Jia Youpeng; Chen Aona; Xie Yayun; Huang Jian; Li Zheng; Nie Gang; Liu Hui; Liu Fuchen; Gao Wenchao;

  • 作者单位

    Second Mil Med Univ Changzheng Hosp Dept Gen Surg 1 Shanghai 200438 Peoples R China;

    Second Mil Med Univ Changzheng Hosp Dept Gen Surg 1 Shanghai 200438 Peoples R China;

    Dalian Municipal Ctr Hosp Gen Surg Dept Dalian Liaoning Peoples R China;

    Second Mil Med Univ Changzheng Hosp Dept Gen Surg 1 Shanghai 200438 Peoples R China;

    Second Mil Med Univ Changzheng Hosp Dept Gen Surg 1 Shanghai 200438 Peoples R China;

    Second Mil Med Univ Eastern Hepatobiliary Surg Hosp Dept Hepat Surg 3 Shanghai 200438 Peoples R;

    Second Mil Med Univ Eastern Hepatobiliary Surg Hosp Dept Hepat Surg 3 Shanghai 200438 Peoples R;

    Second Mil Med Univ Changhai Hosp Dept Hepatobiliary Pancreat Surg Shanghai Peoples R China;

    Second Mil Med Univ Eastern Hepatobiliary Surg Hosp Dept Hepat Surg 3 Shanghai 200438 Peoples R;

    Second Mil Med Univ Eastern Hepatobiliary Surg Hosp Dept Hepat Surg 3 Shanghai 200438 Peoples R;

    Second Mil Med Univ Changzheng Hosp Dept Gen Surg 1 Shanghai 200438 Peoples R China;

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  • 正文语种 eng
  • 中图分类 外科学;
  • 关键词

    CRC; LN metastasis; nomogram; the LASSO regression; logistic regression model; RNA-seq;

    机译：CRC;LN转移;罗斯图;套索回归;Logistic回归模型;RNA-SEQ;

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