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首页> 外文期刊>Biotechnology Progress >Microfibrous Carriers for Cell Culture: A Comparative Study
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Microfibrous Carriers for Cell Culture: A Comparative Study

机译：细胞培养的微纤维载体：比较研究

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摘要

Small patches of polyethylene terephthalate (PET) nonwoven microfibrous matrices have excellent properties and can be used as carriers for culturing cells in agitated bioreactors. The microfibrous carriers are highly porous and can provide large surface areas and three-dimensional space for high-density cell growth. In this work, the microfibrous carriers and several commercial microcarriers were used to study cell attachment kinetics, growth, and monoclonal antibody production with Chinese hamster ovary cells. Compared with commercial solid and macroporous microcarriers, the microfibrous carriers showed better or similar performances. In addition, the microfibrous carriers provided a wider operable range for agitation rate than commercial microcarriers, effectively protecting cells from shear stress and carrier collisions. In addition, the microfibrous carriers are available at a much lower cost than commercial microcarriers, providing an attractive alternative to microcar-rier-based large-scale cell cultures.
机译：聚对苯二甲酸乙二酯（PET）非织造微纤维基质的小补丁具有出色的性能，可以用作在搅拌的生物反应器中培养细胞的载体。微纤维载体是高度多孔的并且可以提供大的表面积和三维空间用于高密度细胞生长。在这项工作中，微纤维载体和几种市售的微载体被用来研究中国仓鼠卵巢细胞的细胞粘附动力学，生长和单克隆抗体的产生。与市售的固体和大孔微载体相比，微纤维载体表现出更好或相似的性能。另外，微纤维载体提供了比商业微载体更大的搅拌速率可操作范围，有效地保护细胞免受剪切应力和载体碰撞。另外，与商业微载体相比，微纤维载体的成本要低得多，为基于微载体的大规模细胞培养提供了有吸引力的替代方法。

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