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Biomass Production from Trichoderma viride in Nonconventional Oat Medium

机译：非常规燕麦培养基中绿色木霉的生物质生产

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摘要

Oatmeal, an alternative, renewable, and low-cost substrate, was used for the production of Trichoderma viride spores by submerged fermentation. The nonconventional oat medium was only supplemented with potato peptone, which is a green source of nitrogen for the microorganism. Because particles are suspended in the nonconventional oat medium, the characterization was based on viscosity, average particle diameter, size distribution, and porosity of the particles. Because of the complexity of the fungal biomass extraction, the dry weight and protein content were used as methods for quantifying the growth of T. viride. The inversion between the proportion of mycelia and spores was captured in the microscopic image analysis during the fermentation process. After 60 h, spores began to appear, accounting for most of the form present at 120 h of fermentation. The decrease in pH and the increase in glucose concentration during fermentation indicate that glucan hydrolysis occurs and that glucose is released into the medium. The potential for industrial applications of submerged fermentation with oats for biomass production of T. viride is noted in the results. This simple and easily controllable process has several advantages, including the use of low-cost substrates for the propagation of a microorganism that is widely used in scientific and commercial settings.
机译：燕麦片是一种可替代的，低成本的可再生底物，被用于通过浸没式发酵生产木霉木霉菌孢子。非常规燕麦培养基仅补充了马铃薯蛋白one，马铃薯蛋白for是微生物的绿色氮源。由于颗粒悬浮在非常规燕麦介质中，因此表征基于颗粒的粘度，平均粒径，尺寸分布和孔隙率。由于真菌生物质提取的复杂性，干重和蛋白质含量被用作定量棉铃虫生长的方法。在发酵过程中的显微图像分析中捕获了菌丝体和孢子比例之间的倒置。 60小时后，孢子开始出现，占发酵120小时时存在的大部分形式。发酵过程中pH的降低和葡萄糖浓度的增加表明发生了葡聚糖水解，并且葡萄糖释放到了培养基中。结果表明，用燕麦进行的深层发酵工业应用具有潜在的生物多样性，可用于生产三叶草。这种简单且易于控制的过程具有多个优点，包括使用低成本的底物来繁殖在科学和商业环境中广泛使用的微生物。

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