Structural characterizations of lipids A by MS/MS of doubly charged ions on a hybrid linear ion trap/orbitrap mass spectrometer

Silipo A; De Castro C; Lanzetta R; Molinaro A; Parrilli M; Vago G; Sturiale L; Messina A; Garozzo D

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Structural characterizations of lipids A by MS/MS of doubly charged ions on a hybrid linear ion trap/orbitrap mass spectrometer

机译：脂合带离子MS / MS在杂交线性离子阱/绕组质谱仪上的脂质A的结构特征

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Here, a new 'one pot' and fast approach is described, based on electrospray ionization (ESI) of negative ions by using a hybrid linear ion trap/orbitrap mass spectrometer (LTQ/orbitrap) for MS and MS/MS analysis. By this method the distribution of the primary and secondary acyl residues of the intact lipid A is inferred by analysis of the ESI spectra measured in positive and negative mode. The analysis of these data allows an unequivocal assignment of the fatty acid distribution. This methodology was successfully tested on two different lipid A with known structures, deriving from the Agrobacterium tumefaciens and Escherichia coli lipopolysaccharides (LPS).

机译：这里，通过使用用于MS和MS / MS / MS分析的混合线性离子阱/轨道质谱仪（LTQ / orbitrap），基于负离子的电喷雾电离（ESI）来描述新的“一个罐”和快速方法。通过该方法，通过以正和阴性模式测量的ESI光谱分析推断完整脂质A的初级和次级酰基残基的分布。对这些数据的分析允许脂肪酸分布的明确分配。该方法在两种不同的脂质A上成功地测试了具有已知结构的，衍生自Tumefaciens和大肠杆菌脂多糖（LPS）。

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来源
《Journal of mass spectrometry: JMS》 |2008年第4期|共7页
作者
Silipo A; De Castro C; Lanzetta R; Molinaro A; Parrilli M; Vago G; Sturiale L; Messina A; Garozzo D;
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作者单位

Univ Naples Federico 2 Dipartimento Chim Organ &

Biochim I-80126 Naples Italy.;

Univ Naples Federico 2 Dipartimento Chim Organ &

Biochim I-80126 Naples Italy.;

Univ Naples Federico 2 Dipartimento Chim Organ &

Biochim I-80126 Naples Italy.;

Univ Naples Federico 2 Dipartimento Chim Organ &

Biochim I-80126 Naples Italy.;

Univ Naples Federico 2 Dipartimento Chim Organ &

Biochim I-80126 Naples Italy.;

Thermo Electron SpA I-20090 Rodano Milano Italy.;

CNR Ist Chim &

Tecnol Polimeri Catania I-95125 Catania Italy.;

CNR Ist Chim &

Tecnol Polimeri Catania I-95125 Catania Italy.;

CNR Ist Chim &

Tecnol Polimeri Catania I-95125 Catania Italy.;

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原文格式 PDF
正文语种 eng
中图分类分析化学;
关键词
lipid A; MS/MS; orbitrap; endotoxins; lipopolysaccharides;

机译：脂质A;MS / MS;orbitrap;内毒素;脂多糖;

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专利

1. Structural characterizations of lipids A by MS/MS of doubly charged ions on a hybrid linear ion trap/orbitrap mass spectrometer [J] . Silipo A, De Castro C, Lanzetta R, Journal of mass spectrometry: JMS . 2008,第4期

机译：脂合带离子MS / MS在杂交线性离子阱/绕组质谱仪上的脂质A的结构特征
2. Simultaneous determination of 20 trace organic chemicals in waters by solid-phase extraction (SPE) with triple-quadrupole mass spectrometer (QqQ-MS) and hybrid quadrupole Orbitrap high resolution MS (Q-Orbitrap-HRMS) [J] . Chen Wei, Huang Huanfang, Chen Chang-Er, Chemosphere . 2016,第nova期

机译：使用三重四极杆质谱仪（QqQ-MS）和混合四极杆Orbitrap高分辨率MS（Q-Orbitrap-HRMS）固相萃取（SPE）同时测定水中20种痕量有机化学物质
3. Structural characterization of in vitro rat liver microsomal metabolites of antihistamine desloratadine using LTQ-Orbitrap hybrid mass spectrometer in combination with online hydrogen/deuterium exchange HR-LC/MS [J] . Chen GD, Daaro I, Pramanik BN, Journal of mass spectrometry: JMS . 2009,第2期

机译：LTQ-Orbitrap混合质谱仪结合在线氢/氘交换HR-LC / MS对体外抗组胺去氯雷他定大鼠肝微粒体代谢产物的结构表征
4. Higher resolution LC-MS and MS-MS analysis of lipid extracts using benchtop Orbitrap-based mass spectrometers and LipidSearch software [C] . Yingying Huang, David Peake, Reiko Kiyonami, International Mass Spectrometry Conference . 2014

机译：使用基于Benchtop orbitrap的质谱仪和Lipidsearch软件的高分辨率LC-MS和脂质提取物的MS-MS分析
5. Fourier transform ion cyclotron resonance mass spectrometry instrumentation and methods for structural characterization of trapped biomolecular ions: Innovative MS/MS techniques, gas-phase hydrogen /deuterium exchange, and laser -induced fluorescence. [D] . McFarland, Melinda A. 2004

机译：傅里叶变换离子回旋共振质谱仪和捕获的生物分子离子的结构表征方法：创新的MS / MS技术，气相氢/氘交换和激光诱导的荧光。
6. Ultra High Resolution Linear Ion Trap Orbitrap Mass Spectrometer (Orbitrap Elite) Facilitates Top Down LC MS/MS and Versatile Peptide Fragmentation Modes [O] . Annette Michalski, Eugen Damoc, Oliver Lange, 2012

机译：超高分辨率线性离子阱Orbitrap质谱仪（Orbitrap Elite）有助于自上而下的LC MS / MS和多功能肽片段化模式
7. Ultra High Resolution Linear Ion Trap Orbitrap Mass Spectrometer (Orbitrap Elite) Facilitates Top Down LC MS/MS and Versatile Peptide Fragmentation Modes* [O] . Michalski, Annette, Damoc, Eugen, Lange, Oliver, 2011

机译：超高分辨率线性离子阱Orbitrap质谱仪（Orbitrap Elite）促进了自上而下的LC MS / MS和多功能肽片段化模式*
8. Miniaturized GC/MS instrumentation for in situ measurements: micro gas chromatography coupled with miniature quadrupole array and paul ion trap mass spectrometers [R] . Holland, P., Chutjian, A., Darrach, M., 2002

机译：用于原位测量的小型化GC / ms仪器：微型气相色谱仪与微型四极杆阵列和保留离子阱质谱仪相结合

Structural characterizations of lipids A by MS/MS of doubly charged ions on a hybrid linear ion trap/orbitrap mass spectrometer

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