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Estimating the contribution of surfactant replacement therapy to the alveolar pool: An in vivoin vivo study based on 1313 C natural abundance in rabbits

机译：估算表面活性剂替代疗法对肺泡池的贡献：基于 ：//www.wiley.com/namespaces/wiley“> 13 13 c在兔子中的天然丰富

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Abstract > Variation of the isotopic abundance of selected nutrients and molecules has been used for pharmacological and kinetics studies under the premise that the administered molecule has a different isotopic enrichment from the isotopic background of the recipient subject. The aim of this study is to test the feasibility of assessing the contribution of exogenous surfactant phospholipids to the endogenous alveolar pool in vivo after exogenous surfactant replacement therapy in rabbits. > The study consisted in measuring the consistency of 13 C/ 12 C ratio of disaturated‐phosphatidylcholine palmitate (DSPC‐PA) in 7 lots of poractant alfa, produced over a year, and among bronchoalveolar lavages of 20 rabbits fed with a standard chow. A pilot study was performed in a rabbit model of lavage‐induced surfactant deficiency: 7 control rabbits and 4 treated with exogenous surfactant. The contribution of exogenous surfactant to the alveolar pool was assessed after intra‐tracheal administration of 200?mg/kg of poractant alfa. The 13 C content of DSPC‐PA was measured by isotope ratio mass spectrometry. > The mean DSPC‐PA 13 C/ 12 C ratio of the 7 lots of poractant alfa was ?18.8‰ with a SD of 0.1‰ (range: ?18.9‰; ?18.6‰). The mean 13 C/ 12 C ratio of surfactant DSPC recovered from the lung lavage of 20 rabbits was ?28.8?±?1.2‰ (range: ?31.7‰; ?25.7‰). The contribution of exogenous surfactant to the total alveolar surfactant could be calculated in the treated rabbits, and it ranged from 83.9% to 89.6%. > This pilot st </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >变体所选择的营养素和分子的同位素丰度已用于药理学和动力学研究的前提下，给药的分子与受体受体受试者的同位素背景具有不同的同位素富集。本研究的目的是测试评估外源表面活性剂磷脂对内源性肺泡池中的贡献的可行性在兔子外源表面活性剂替代治疗后体内含量肺泡池中的含量。该研究包括测量7批分泌术中的杂质磷脂酰胆碱棕榈酸盐（DSPC-PA）的 13℃/ sup> 12 </ sup> c比率的一致性Alfa，一年多发生产，其中20只兔子的支气管肺泡灌洗液喂养标准咸菜。在灌洗诱导的表面活性剂缺乏症的兔模型中进行了试验研究：7种对照兔和用外源表面活性剂处理的4。在气管内施用200μmMg/ kg术治疗术后，评估外源表面活性剂对肺泡池的贡献。通过同位素比质谱法测量DSPC-PA的 13 / sup> c含量。 </ p> >平均dspc-pa 13 </ sup> c / suc> 12 </ sup> c比率的7批分泌者Alfa为18.8，SD为0.1‰（范围：18.9‰;？18.6‰）。从20只兔子的肺灌洗中恢复的表面活性剂DSPC的平均值 13℃/ 12 </ sup> c比率是α28.8？±1.2°（范围：？31.7‰;？25.7 ‰）。外源表面活性剂对总肺泡表面活性剂的贡献可以在处理的兔子中计算，其范围为83.9％至89.6％。 </ p> >这个pilot st </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-34906/'>《Journal of mass spectrometry: JMS》</a> <b style="margin: 0 2px;">|</b><span>2018年第7期</span><b style="margin: 0 2px;">|</b><span>共5页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Giambelluca Sonia&option=202" target="_blank" rel="nofollow">Giambelluca Sonia;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ricci Francesca&option=202" target="_blank" rel="nofollow">Ricci Francesca;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Simonato Manuela&option=202" target="_blank" rel="nofollow">Simonato Manuela;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Correani Alessio&option=202" target="_blank" rel="nofollow">Correani Alessio;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Casiraghi Costanza&option=202" target="_blank" rel="nofollow">Casiraghi Costanza;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Storti Matteo&option=202" target="_blank" rel="nofollow">Storti Matteo;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cogo Paola&option=202" target="_blank" rel="nofollow">Cogo Paola;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Salomone Fabrizio&option=202" target="_blank" rel="nofollow">Salomone Fabrizio;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Carnielli Virgilio Paolo&option=202" target="_blank" rel="nofollow">Carnielli Virgilio Paolo;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Women's and Children's HealthPadova University HospitalPadova Italy;</p> <p>R&</p> <p>D DepartmentChiesi FarmaceuticiParma Italy;</p> <p>Division of Pediatrics Department of MedicineUniversity of UdineUdine Italy;</p> <p>Division of Neonatology Department of Clinical SciencesPolytechnic University of Marche and Azienda Ospedaliero‐Universitaria Ospedali RiunitiAncona Italy;</p> <p>R&</p> <p>D DepartmentChiesi FarmaceuticiParma Italy;</p> <p>R&</p> <p>D DepartmentChiesi FarmaceuticiParma Italy;</p> <p>Division of Pediatrics Department of MedicineUniversity of UdineUdine Italy;</p> <p>R&</p> <p>D DepartmentChiesi FarmaceuticiParma Italy;</p> <p>Division of Neonatology Department of Clinical SciencesPolytechnic University of Marche and Azienda Ospedaliero‐Universitaria Ospedali RiunitiAncona Italy;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=13 C natural abundance&option=203" rel="nofollow">13 C natural abundance;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=GC‐C‐IRMS&option=203" rel="nofollow">GC‐C‐IRMS;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=RDS&option=203" rel="nofollow">RDS;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=stable isotope&option=203" rel="nofollow">stable isotope;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=surfactant&option=203" rel="nofollow">surfactant;</a> </p> <div class="translation"> 机译：13 c天然丰富;GC-C-IRMS;RDS;稳定同位素;表面活性剂; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025633337.html">Estimating the contribution of 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