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首页> 外文期刊>Biotechnology Progress >Galacto-OIigosaccharides and Lactulose as Protectants Against Desiccation of Lactobacillus delbrueckii subsp. bulcaricus
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Galacto-OIigosaccharides and Lactulose as Protectants Against Desiccation of Lactobacillus delbrueckii subsp. bulcaricus

机译：半乳寡糖和乳果糖作为防止德氏乳杆菌亚种干燥的保护剂。保加利亚

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摘要

Lactobacillus delbrueckii subsp. bulgaricus CIDCA 333 was dehydrated on desiccators containing silica gel in the presence of 20% wlw of two types of galacto-oligosaccharides (GOS Biotempo and GOS Cup Oligo H-70~R) and lactulose, until no changes in water desorption were detected. After rehydration, bacterial growth was monitored at 37°C by determining: (a) the absorbance at 600 nm and (b) the near infrared spectra (NIR). Principal component analysis (PCA) was then performed on the NIR spectra of samples dehydrated in all conditions. A multiparametric flow cytometry assay was carried out using carboxyfluorescein diacetate and propidium iodide probes to determine the relative composition of damaged, viable, and dead bacteria throughout the growth kinetics. The absorbance at 600 nm and the position of the second derivative band at ~1370 nm were plotted against the time of incubation. The efficiency of the protectants was GOS Biotempo >GOS Cup Oligo H-70~R >lactulose. The better protectant capacity of GOS Biotempo was explained on the basis of the lower contribution of damaged cells immediately after rehydration (t = 0). PC A showed three groups along PCI, corresponding to the lag, exponential and stationary phases of growth, which explained 99% of the total variance. Along PC2, two groups were observed, corresponding to damaged or viable cells. The results obtained support the use of NIR to monitor the recovery of desiccated microorganisms in real time and without the need of chemical reagents. The use of GOS and lactulose as protectants in dehydration/rehydration processes was also supported.
机译：德氏乳杆菌亚种。在20％wlw的两种类型的低聚半乳糖（GOS Biotempo和GOS Cup Oligo H-70〜R）和乳果糖的存在下，将保加利亚CIDCA 333在含有硅胶的干燥剂上脱水，直到未检测到水解吸变化为止。补液后，通过确定：（a）在600 nm处的吸光度和（b）近红外光谱（NIR）来监测细菌在37°C下的生长。然后对在所有条件下脱水的样品的近红外光谱进行主成分分析（PCA）。使用羧基荧光素二乙酸酯和碘化丙啶探针进行了多参数流式细胞术测定，以确定在整个生长动力学中受损，存活和死亡细菌的相对组成。相对于孵育时间绘制600nm处的吸光度和〜1370nm处的二阶导数带的位置。保护剂的效率为：GOS Biotempo> GOS Cup Oligo H-70〜R>乳果糖。 GOS Biotempo具有更好的保护能力，其解释是基于补液后立即受损细胞的较低贡献（t = 0）。 PC A沿PCI显示三组，分别对应于增长的滞后，指数和平稳阶段，这解释了总方差的99％。沿着PC2，观察到两组，分别对应于受损或存活的细胞。获得的结果支持使用NIR实时监测干燥微生物的回收，而无需化学试剂。还支持在脱水/脱水过程中使用GOS和乳果糖作为保护剂。

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