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首页> 外文期刊>Journal of Medical Entomology >Comparative Mitogenomic Analysis of Forensically Important Sarcophagid Flies (Diptera: Sarcophagidae) and Implications of Species Identification
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Comparative Mitogenomic Analysis of Forensically Important Sarcophagid Flies (Diptera: Sarcophagidae) and Implications of Species Identification

机译：对比重要的嗜碱性嗜酸虫（Diptera：sarcophagidae）和物种鉴定的影响

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摘要

The flesh flies (Diptera: Sarcophagidae) are significant in forensic investigations. The mitochondrial genome (mitogeome) has been widely used as genetic markers for phylogenetic analysis and species identification. To further understand the mitogenome-level features in Sarcophagidae, the complete mitogenome of Sarcophaga formosensis (Kirneret Lopes, 1961) (Diptera: Sarcophagidae) and Sarcophaga misera (Walker, 1849) (Diptera: Sarcophagidae) was firstly sequenced, annotated, and compared with other 13 Sarcophagidae species. The result indicated that the gene arrangement, gene content, base composition, and codon usage were conserved in the ancestral arthropod. Evolutionary rate of the mitogenome fragments revealed that the nonsynonymous and synonymous substitution rates (Ka and Ks) ratio was less than 1.00, indicating these variable sites under strong purifying selection. Almost all transfer RNA genes (tRNAs) have typical clover-leaf structures within these sarcophagid mitogenomes, except tRNA-Ser (AGN) is lack of the dihydrouridine arm. This comparative mitogenomic analysis sheds light on the architecture and evolution of mitogenomes in the Sarcophagidae. Phylogenetic analyses containing the interspecific distances from different regions in these species provided us new insights into the application of these effective genetic markers for species identification of flesh flies.
机译：肉体苍蝇（Diptera：sarcophagidae）在法医调查中是显着的。线粒体基因组（米科族）已被广泛用作系统发育分析和物种鉴定的遗传标志物。为了进一步了解Sarcophagidae的促催化剂型特征，Sarcophaga Formosensis（Kirneret Lopes，1961）（Diptera：Sarcophagidae）和Sarcophaga Misera（Walker，1849）（Diptera：Sarcophagidae）的完整促使促进型催乳素组（Dippera：Sarcophagidae）与其他13种石棺。结果表明，在祖先节肢动物中，基因排列，基因含量，基础组成和密码子使用。促使碎片的进化率揭示了非同义词和同义词替代率（Ka和Ks）比率小于1.00，表明这些可变位点在强净化选择下。几乎所有转移RNA基因（TrNAS）都有在这些酸化毒蛛内具有典型的三叶草结构，但是除了缺乏二氢鲁法臂之外。这种比较诱导的方法分析揭示了石棺中毒蛛的建筑和演变。含有这些物种中不同区域的三个距离的系统发育分析为美国提供了新的见解，这些有效遗传标志物用于物种鉴定肉苍蝇。

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