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首页> 外文期刊>Biotechnology Progress >On-Line Optical Fiber Detection in a Preparative Free-Flow Electrofocusing Apparatus
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On-Line Optical Fiber Detection in a Preparative Free-Flow Electrofocusing Apparatus

机译：制备自由流电聚焦设备中的在线光纤检测

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Many analytical isoelectric focusing (IEF)instruments are equipped with on-line detection,e.g.,conductivity or UV-vis absorbance.Most of their preparative counterparts have not integrated such detection systems and thus require labor-intensive off-line analysis to quantify separation results.This paper describes the incorporation of an optical fiber based,on-line detection system that allows one to follow the evolution of the protein bands in a preparative IEF apparatus.An array of four optical fibers was designed to deliver light to the annulus of a free-flow electrophoresis apparatus,to detect the transmitted light passing through the separation media and to determine the protein concentration at vertical positions along the annulus of a vortex-stabilized focusing chamber using a 1024 bit CCD line camera.The final concentration of the major myoglobin band was 21.0 mg/mL at electric field strengths as high as 333 V/cm.Spectrophotometric analysis indicated a final concentration of 18.9 mg/mL,10% less than that reported by the optical fibers.
机译：许多分析等电聚焦（IEF）仪器都配备了在线检测功能，例如电导率或UV-vis吸光度。大多数制备型对等设备尚未集成此类检测系统，因此需要劳动密集型离线分析来量化分离结果本文介绍了一种基于光纤的在线检测系统的结合，该系统可以跟踪制备型IEF装置中蛋白质条带的演变。设计了四根光纤阵列，可将光传输到椎间盘环自由流动电泳仪，使用1024位CCD线相机检测穿过分离介质的透射光，并确定沿涡旋稳定聚焦室环空垂直位置的蛋白质浓度。主要肌红蛋白的最终浓度电场强度高达333 V / cm时，谱带为21.0 mg / mL。分光光度分析表明终浓度为18.9 mg / mL，比光纤报告的低10％。

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