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首页> 外文期刊>Biotechnology Progress >Aptamer-Mediated Magnetic and Gold-Coated Magnetic Nanoparticles as Detection Assay for Prion Protein Assessment
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Aptamer-Mediated Magnetic and Gold-Coated Magnetic Nanoparticles as Detection Assay for Prion Protein Assessment

机译：适体介导的磁性和金包被的磁性纳米粒子作为Pri蛋白评估的检测方法

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摘要

This article reports the chemical synthesis and functionalization of magnetic and gold-coated magnetic nanoparticles.The binding characteristics of streptavidin-conjugated nanoparticles were studied using prion protein as a target to a specific biotinylated aptamer.The size and structure of the particles were determined by transmission electron microscopy,and the binding of the prion to the particle was confirmed by Fourier transform infrared spectroscopy.The rate of prion binding to the aptamer was dose-dependent,and prion immobilization was more effective on L-aspartic acid-functionalized magnetic nanoparticles compared to the carboxyl-functionahzed gold-coated magnetic nanoparticles.This study sets the stage for the development of prion detection platforms as well as our long-term goals in structure elucidation at the binding interface.
机译：本文报道了磁性和金包被的磁性纳米粒子的化学合成和功能化。以病毒蛋白为靶标，研究了链霉亲和素缀合的纳米粒子与特定生物素化适体的结合特性，通过透射确定了粒子的大小和结构电子显微镜检查，并通过傅立叶变换红外光谱法证实了ion病毒与粒子的结合。of病毒与适体的结合速率是剂量依赖性的，与L-天冬氨酸官能化的磁性纳米粒子相比，病毒的固定更有效这项研究为病毒检测平台的开发以及我们在结合界面的结构阐明的长期目标奠定了基础。

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