Hepatitis B virus X protein blocks filamentous actin bundles by interaction with eukaryotic translation elongat ion factor 1 alpha 1

LinW.-S.; JiaoB.-Y.; WuY.-L.; ChenW.-N.; LinX.

首页> 外文期刊>Journal of Medical Virology >Hepatitis B virus X protein blocks filamentous actin bundles by interaction with eukaryotic translation elongat ion factor 1 alpha 1

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Hepatitis B virus X protein blocks filamentous actin bundles by interaction with eukaryotic translation elongat ion factor 1 alpha 1

机译：乙型肝炎病毒X蛋白通过与真核翻译Elongat离子因子1α1的相互作用阻断丝状肌动蛋白束

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Hepatitis B virus (HBV)-encoded X protein (HBx protein) is a multi-functional regulatory protein. It functions by protein-protein interaction and plays a pivotal role in the pathogenesis of HBV-related diseases. However, the partners in hepatocytes interacting with HBx protein are far from understood fully. In this study, immunoprecipitation was employed to screen for binding partners for the HBx protein from huh-7 hepatoma cells infected with recombinant adenovirus expressing HBx protein, and five cellular proteins including eukaryotic translation elongation factor 1 alpha 1 (eEF1A1), were identified. The interaction between HBx protein and eEF1A1 was confirmed further using a GST pull-down assay and co-immunoprecipitation, respectively. In Huh-7 hepatoma cells, the HBx protein inhibits dimer formation of eEF1A1, hence blocks filamentous actin bundling. These findings provide new insights into the molecular mechanisms involved in the functions of the HBx protein.

机译：乙型肝炎病毒（HBV） - 索尼酸X蛋白（HBX蛋白）是一种多功能调节蛋白。它通过蛋白质 - 蛋白质相互作用起作用，并在HBV相关疾病的发病机制中发挥枢轴作用。然而，与HBX蛋白相互作用的肝细胞中的伴侣远非完全理解。在该研究中，使用免疫沉淀到来自Huh-7 HBX蛋白的HBX蛋白的结合伴侣，其来自表达HBX蛋白的重组腺病毒的HUH-7肝癌细胞，并且鉴定了包括真核转化伸长因子1（EEF1A1）的五种细胞蛋白质。使用GST下拉测定和共免疫沉淀，进一步确认HBX蛋白和EEF1A1之间的相互作用。在HUH-7肝癌细胞中，HBX蛋白抑制EEF1A1的二聚体形成，因此阻断丝状肌动蛋白捆扎。这些发现提供了新的见解，进入HBX蛋白的功能的分子机制。

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来源
《Journal of Medical Virology》 |2012年第6期|共7页
作者
LinW.-S.; JiaoB.-Y.; WuY.-L.; ChenW.-N.; LinX.;
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作者单位

Key Laboratory of Ministry of Education for Gastrointestinal Cancer Research Center of Molecular;

Key Laboratory of Ministry of Education for Gastrointestinal Cancer Research Center of Molecular;

Key Laboratory of Ministry of Education for Gastrointestinal Cancer Research Center of Molecular;

Key Laboratory of Ministry of Education for Gastrointestinal Cancer Research Center of Molecular;

Key Laboratory of Ministry of Education for Gastrointestinal Cancer Research Center of Molecular;

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原文格式 PDF
正文语种 eng
中图分类医学微生物学（病原细菌学、病原微生物学）;
关键词
Eukaryotic translation elongation factor 1 alpha 1; Hepatitis B virus; Immunoprecipitation;

机译：真核翻译伸长因子1α1;乙型肝炎病毒;免疫沉淀;

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专利

1. Hepatitis B virus X protein blocks filamentous actin bundles by interaction with eukaryotic translation elongat ion factor 1 alpha 1 [J] . LinW.-S., JiaoB.-Y., WuY.-L., Journal of Medical Virology . 2012,第6期

机译：乙型肝炎病毒X蛋白通过与真核翻译延伸因子1 alpha 1相互作用而阻断丝状肌动蛋白束
2. Eukaryotic Initiation Factor 4G-Poly(A) Binding Protein Interaction Is Required for Poly(A) Tail-Mediated Stimulation of Picornavirus Internal Ribosome Entry Segment-Driven Translation but Not for X-Mediated Stimulation of Hepatitis C Virus Translation [J] . Yanne M. Michel, Andrew M. Borman, Sylvie Paulous, Molecular and Cellular Biology . 2001,第13期

机译：真核生物起始因子4G-Poly（A）结合蛋白相互作用是小核糖核酸病毒内部核糖体进入片段驱动翻译的Poly（A）尾部介导的刺激，而不是丙型肝炎病毒翻译的X介导的刺激。
3. Tetrahymena eukaryotic translation elongation factor 1A (eEF1A) bundles filamentous actin through dimer formation. [J] . Bunai F, Ando K, Ueno H, The Journal of Biochemistry . 2006,第3期

机译：四膜虫真核翻译延伸因子1A（eEF1A）通过二聚体形成捆绑丝状肌动蛋白。
4. Computational Identification of Interaction Motifs in Hepatitis C Virus NS5A and Human Proteins [C] . Guang-Zheng Zhang, Kyungsook Han Convergence Information Technology (ICCIT), 2007 International Conference on . 2007

机译：丙型肝炎病毒NS5A与人类蛋白质相互作用基序的计算鉴定
5. Interactions of eukaryotic translation initiation factors and 3' untranslated region of barley yellow dwarf virus mRNA during protein synthesis: A study of equilibrium binding, kinetics and thermodynamics. [D] . Banerjee, Bidisha. 2014

机译：蛋白质合成过程中大麦黄矮病毒mRNA的真核翻译起始因子和3'非翻译区的相互作用：平衡结合，动力学和热力学的研究。
6. Eukaryotic Initiation Factor 4G-Poly(A) Binding Protein Interaction Is Required for Poly(A) Tail-Mediated Stimulation of Picornavirus Internal Ribosome Entry Segment-Driven Translation but Not for X-Mediated Stimulation of Hepatitis C Virus Translation [O] . Yanne M. Michel, Andrew M. Borman, Sylvie Paulous, 2001

机译：真核生物起始因子4G-Poly（A）结合蛋白相互作用是小核糖核酸病毒内部核糖体进入片段驱动翻译的Poly（A）尾部介导刺激的必需条件但对于丙型肝炎病毒翻译的X介导刺激则不需要
7. Eukaryotic Initiation Factor 4G-Poly(A) Binding Protein Interaction Is Required for Poly(A) Tail-Mediated Stimulation of Picornavirus Internal Ribosome Entry Segment-Driven Translation but Not for X-Mediated Stimulation of Hepatitis C Virus Translation [O] . Michel, Yanne M., Borman, Andrew M., Paulous, Sylvie, 2001

机译：真核生物起始因子4G-Poly（A）结合蛋白相互作用是小核糖核酸病毒内部核糖体进入片段驱动翻译的Poly（A）尾部介导刺激的必需条件，但对于丙型肝炎病毒翻译的X介导刺激则不需要
8. Hantaan Virus Nucleocapsid Protein Binds to Importin alpha Proteins and Inhibits Tumor Necrosis Factor Alpha-Induced Activation of Nuclear Factor Kappa B [R] . Taylor, S. L., Frias-Staheli, N., Garcia-Sastre, A., 2008

机译：汉坦病毒核衣壳蛋白与进口蛋白α蛋白结合并抑制肿瘤坏死因子α诱导的核因子κB活化

Hepatitis B virus X protein blocks filamentous actin bundles by interaction with eukaryotic translation elongat ion factor 1 alpha 1

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