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首页> 外文期刊>Journal of Medical Virology >Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens
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Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens

机译：使用常规临床呼吸标本进行新的自动核酸提取系统的前瞻性评价

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The aim of the study was to evaluate the MagNA Pure 96? nucleic acid extraction system using clinical respiratory specimens for identifying viruses by qualitative real-time PCR assays. Three extraction methods were tested, that is, the MagNA Pure LC?, the COBAS Ampliprep?, and the MagNA Pure 96? with 10-fold dilutions of an influenza A(H1N1)pdm09 sample. Two hundred thirty-nine respiratory specimens, 35 throat swabs, 164 nasopharyngeal specimens, and 40 broncho-alveolar fluids, were extracted with the MagNA Pure 96? and the COBAS Ampliprep? instruments. Forty COBAS Ampliprep? positive samples were also tested. Real-time PCRs were used to identify influenza A and influenza A(H1N1)pdm09, rhinovirus, enterovirus, adenovirus, varicella zoster virus, cytomegalovirus, and herpes simplex virus. Similar results were obtained on RNA extracted from dilutions of influenza A(H1N1)pdm09 with the three systems: the MagNA Pure LC?, the COBAS Ampliprep?, and the MagNA Pure 96?. Data from clinical respiratory specimens extracted with the MagNA Pure 96? and COBAS Ampliprep? instruments were in 98.5% in agreement (P<0.0001) for influenza A and influenza A(H1N1)pdm09. Data for rhinovirus were in 97.3% agreement (P<0.0001) and in 96.8% agreement for enterovirus. They were in 100% agreement for adenovirus. Data for cytomegalovirus and HSV1-2 were in 95.2% agreement (P<0.0001). The MagNA Pure 96? instrument is easy-to-use, reliable, and has a high throughput for extracting total nucleic acid from respiratory specimens. These extracts are suitable for molecular diagnosis with any type of real-time PCR assay.
机译：该研究的目的是评估MAGNA纯96次？核酸提取系统使用临床呼吸试样通过定性实时PCR测定来鉴定病毒。测试了三种提取方法，即大麦纯lc？，钴族基地纯净β，和麦格纳纯96？具有10倍稀释的流感A（H1N1）PDM09样品。用麦格纳纯96提取两百三十九个呼吸样品，35次喉咙，164个鼻咽标本和40个支气管 - 肺泡液体。和COBAS AMPLIPREP？仪器。四十个COBAS AMPLIPREP？还测试了阳性样品。实时PCR用于鉴定流感A和流感A（H1N1）PDM09，鼻病毒，肠道病毒，腺病毒，水痘系罗斯病毒，巨细胞病毒和单纯疱疹病毒。在用三种系统中从稀释液中提取的RNA提取了类似的结果：MAGNA纯LC？，COBAS SAMPRIPREP？，MAGNA纯96℃。用麦格纳纯96提取临床呼吸样品的数据？和雪橇放大器？仪器在协议（P <0.0001）中为98.5％，用于流感A和流感A（H1N1）PDM09。鼻病毒的数据均为97.3％协议（P <0.0001）和96.8％的肠病达成协议。它们是腺病毒100％的协议。细胞瘤病毒和HSV1-2的数据均为95.2％协议（P <0.0001）。麦格纳纯96？仪器易于使用，可靠，并且具有高通量，用于从呼吸样品中提取总核酸。这些提取物适用于具有任何类型的实时PCR测定的分子诊断。

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