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首页> 外文期刊>Journal of Medical Virology >Characterization of the nasopharyngeal viral microbiome from children with community-acquired pneumonia but negative for Luminex xTAG respiratory viral panel assay detection
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Characterization of the nasopharyngeal viral microbiome from children with community-acquired pneumonia but negative for Luminex xTAG respiratory viral panel assay detection

机译:患有社区获得性肺炎的儿童的鼻咽病毒微生物瘤的特征,但对Luminex XTAG呼吸道病毒组测定检测的阴性

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摘要

In the present study, 50 nasopharyngeal swabs from children with community-acquired pneumonia (CAP) but negative for 18 common respiratory viruses, as measured by the Luminex xTAG Respiratory Viral Panel Assay, were subjected to multiplex metagenomic analyses using a next-generation sequencing platform. Taxonomic analysis showed that all sequence reads could be assigned to a specific species. An average of 95.13% were assigned to the Bacteria kingdom, whereas, only 0.72% were potentially virus derived. This snapshot of the respiratory tract virome revealed most viral reads to be respiratory tract related, classified into four known virus families: Paramyxoviridae, Herpesviridae, Anelloviridae, and Polyomaviridae. Importantly, we detected a novel human parainfluenza virus 3 (HPIV 3) strain with a 32-bp insertion in the haemagglutinin-neuraminidase (HN) gene that produced a negative result in the Luminex assay, highlighting the strength of virome metagenomic analysis to identify not only novel viruses but also viruses likely to be missed by ordinary clinical tests. Thus, virome metagenomic analysis could become a viable clinical diagnostic method.
机译:在本研究中,通过毫米X XTAG呼吸道病毒组测定测量的来自患有社区获得的肺炎(帽)的50名来自患有社区肺炎(盖子)的鼻咽拭子,但是使用下一代测序平台进行多重偏见分析的多重呼吸道病毒。分类学分析表明,可以将所有序列读数分配给特定物种。将95.13%的平均分配给细菌王国,而只有0.72%的潜在病毒得出。这种呼吸道病毒体的快照显示,大多数病毒读数是呼吸道相关的,分为四个已知的病毒系列:副乳柳虫,苯磺肽,蒽酚和聚瘤。重要的是,我们检测到一种新的人偶然血管病毒3(HPIV 3)菌株,在血红素蛋白 - 神经氨酰胺酶(HN)基因中,在LumminX测定中产生阴性结果,突出了生物组分分析的强度,以识别不辨认只有新型病毒,但普通的临床试验也可能错过的病毒。因此,生物学分析可能成为一种可行的临床诊断方法。

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