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首页> 外文期刊>Journal of molecular histology >Tunneling nanotubes mediate intercellular communication between endothelial progenitor cells and osteoclast precursors
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Tunneling nanotubes mediate intercellular communication between endothelial progenitor cells and osteoclast precursors

机译：隧道纳米管介导内皮祖细胞和骨壳前体之间的细胞间通信

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摘要

Tunneling nanotube (TNT)-mediated cell communication play pivotal roles in a series of physiological and pathological processes in multicellular organism. This study was designed to investigate the existence of TNTs between EPCs and osteoclast precursors and evaluate their effects on the differentiation of osteoclast precursors. For these purposes, EPCs and osteoclast precursors (RAW264.7 cells) were stained with different fluorescent dyes before direct co-culture; then, the co-cultured cells were sorted by fluorescence activated cell sorter (FACS), and the differentiation of co-cultured RAW264.7 cells was evaluated. The results showed that the differentiation potential of RAW264.7 cells was significantly inhibited after their co-culture with EPCs. Additionally, the expression of macrophage migration inhibitory factor (MIF) was up-regulated in RAW264.7 cells after co-culture. Moreover, the MIF inhibitor ISO-1 could rescue the formation of TRAP-positive multinuclear osteoclasts and the expression of osteoclastogenesis-associated genes in the co-cultured RAW264.7 cells. The present study demonstrates that EPCs can affect the differentiation of osteoclast precursors through the TNT-like structures formed across these two types of cells and might inform new therapeutic strategies for osteolytic diseases.
机译：隧道纳米管（TNT）介导的细胞通信在多细胞生物中的一系列生理和病理过程中起枢转作用。本研究旨在探讨EPC和骨壳前体之间TNT的存在，并评估它们对破骨细胞前体的分化的影响。出于这些目的，在直接共培养之前，用不同的荧光染料染色EPC和骨壳前体（RAW264.7细胞）;然后，通过荧光活性细胞分选仪（FAC）对共培养的细胞进行分类，并评估共培养的Raw264.7细胞的分化。结果表明，在与EPC的共同培养后，Raw264.7细胞的分化潜力显着抑制。另外，在共培养后，在Raw264.7细胞中升调巨噬细胞迁移抑制因子（MIF）的表达。此外，MIF抑制剂ISO-1可以拯救捕获阳性多核破骨细胞的形成和在共培养的Raw264.7细胞中形成骨细胞发生相关基因的表达。本研究表明，EPC可以通过在这两种细胞上形成的TNT样结构来影响破骨细胞前体的分化，并可能为骨质溶解疾病提供新的治疗策略。

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