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首页> 外文期刊>Biotechnology Progress >Inhibitory Effects of a Manganese Superoxide Dismutase Isolated from Garlic(Allium Sativum L.)on In Vitro Tumoral Cell Growth
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Inhibitory Effects of a Manganese Superoxide Dismutase Isolated from Garlic(Allium Sativum L.)on In Vitro Tumoral Cell Growth

机译：大蒜（大蒜）中超氧化物歧化酶对体外肿瘤细胞生长的抑制作用

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Reactive oxygen species are implicated in cancer development and antioxidants in general and superoxide dismutases and superoxide dismutase mimetic in particular,and they inhibit malignant transformation.We examinate the effects of an isolated manganese superoxide dismutase from a medicinal plant Allium sativum.The protein was prepared by a serial of chromatographic techniques:gel filtration and diethylaminoethyl ions exchanger.The enzyme has a specific activity equal to 55 U/mg.Two tumoral cell lines,porcine endothelial cells and mouse melanoma cells were exposed to garlic superoxide dismutase.The exogenous manganese superoxide dismutase is able to modify the intracellular level of reactive oxygen species by eliminating superoxide anion and producing hydrogen peroxide.The cell viability of the two lines was not significantly affected but the cell multiplication was arrested.This effect obtained in the presence of manganese superoxide dismutase correlates with the activation and modulation of phospho-extracellular signal-regulated kinases proteins,implicated in the control of several biological processes including cell proliferation.
机译：活性氧与癌症的发展有关，通常与抗氧化剂有关，尤其与超氧化物歧化酶和超氧化物歧化酶模拟物有关，它们抑制恶性转化。我们检验了从药用植物大蒜中分离到的锰超氧化物歧化酶的作用。一系列的色谱技术：凝胶过滤和二乙氨基乙基离子交换剂。该酶的比活性等于55 U / mg。将两个肿瘤细胞系，猪内皮细胞和小鼠黑素瘤细胞暴露于大蒜超氧化物歧化酶。能够通过消除超氧阴离子并产生过氧化氢来改变细胞内活性氧的水平。两个系的细胞活力没有受到明显影响，但细胞增殖被阻止了。在锰超氧化物歧化酶存在下获得的这种效应与激活与模磷酸化细胞外信号调节激酶蛋白的功能，暗示着对包括细胞增殖在内的几种生物学过程的控制。

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