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首页> 外文期刊>Journal of neuro-oncology. >LncRNA GAS5 regulates the proliferation, migration, invasion and apoptosis of brain glioma cells through targeting GSTM3 expression. The effect of LncRNA GAS5 on glioma cells
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LncRNA GAS5 regulates the proliferation, migration, invasion and apoptosis of brain glioma cells through targeting GSTM3 expression. The effect of LncRNA GAS5 on glioma cells

机译：LNCRNA Gas5通过靶向GSTM3表达来调节脑胶质瘤细胞的增殖，迁移，侵袭和凋亡。 LNCRNA气体5对胶质瘤细胞的影响

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Introduction To investigate the effects of lncRNA GAS5 on the proliferation, migration, invasion and apoptosis of brain glioma cells. Methods The expression levels of lncRNA GAS5 and GSTM3 in normal glial cells (HEB) and glioma cells (U251 and U87) were detected by RT-qPCR and western blot, respectively. Glioma cells were transfected with ctrl vector, pcDNA-GAS5, siRNA ctrl (siNC) or GSTM3 siRNA and the effects of lncRNA GAS5 and GSTM3 on the proliferation, migration, invasion and apoptosis of glioma cells were detected by CCK-8 assay, transwell assay and Caspase 3/7 activity assay, respectively. Results The expression of lncRNA GAS5 was significantly decreased in glioma cell lines U251 and U87 compared with normal glial cells HEB (p<0.01). In addition, overexpression of lncRNA GAS5 inhibited the proliferation, migration and invasion of U251 and U87 cells, and promoted cell apoptosis as demonstrated by the increased activity of Caspase 3/7. Furthermore, GSTM3 was predicted as a target gene of lncRNA GAS5 by bioinformatics analysis and its expression was increased in glioma cells compared with the normal cells as indicated by western blotting and RT-qPCR experimental results. Silencing of GSTM3 with GSTM3 siRNA decreased the proliferation, migration and invasion but increased the apoptosis of glioma cell lines U251 and U87, which was similar to that the effect lncRNA GAS5 over-expression. Conclusion lncRNA GAS5 can effectively inhibit the proliferation, migration and invasion of glioma cells and promote cell apoptosis through targeting GSTM3 expression.
机译：介绍LNCRNA气体5对脑胶质瘤细胞增殖，迁移，侵袭和凋亡的影响。方法通过RT-QPCR和Western印迹检测正常胶质细胞（HEB）和胶质瘤细胞（HE251和U87）中LNCRNA气体5和GSTM3的表达水平。通过CCK-8测定，用CTR1载体，PCDNA - 气体5，siRNA Ctrl（sinc）或GSTM3 siRNA和GSTM3 siRNA和GSTM3对胶质瘤细胞的增殖，迁移，侵袭和凋亡的影响转染胶质瘤细胞和GSTM3 siRNA的影响和Caspase 3/7活性测定。结果胶质瘤细胞系U251和U87中LNCRNA气体5的表达显着降低，与正常的胶质细胞HEB（P <0.01）。此外，LNCRNA气体5的过表达抑制U251和U87细胞的增殖，迁移和侵袭，并通过Caspase 3/7的活性增加，促进细胞凋亡。此外，通过生物信息学分析预测GSTM3作为LNCRNA气体5的靶基因，并且与Western印迹和RT-QPCR实验结果表明的正常细胞相比，其表达在胶质瘤细胞中增加。 GSTM3与GSTM3 siRNA的沉默降低了增殖，迁移和侵袭，而是增加了胶质瘤细胞系U251和U87的凋亡，这类似于效果LNCRNA气体5过表达。结论LNCRNA气体5可有效抑制胶质瘤细胞的增殖，迁移和侵袭，并通过靶向GSTM3表达来促进细胞凋亡。

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