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Evaluation of Steam-Treated Giant Bamboo for Production of Fermentable Sugars

机译:蒸制巨竹发酵糖生产的评价

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Giant bamboo plantations are currently being established in the Southern Africa region and can be considered as potential lignocellulosic feedstock for the production of second generation bioethanol. In this study, giant bamboo internodal material was subjected to sulphur dioxide (SO2) impregnated steam pretreatment prior to enzymatic hydrolysis. The effect of temperature, residence time, and acidity on the overall sugar recovery and byproduct formation was studied using response surface response technology according to a central composite experimental design (CCD) at a fixed SO2 concentration of 2.5% (w/w liquid) after impregnation. The results showed that pretreatment conditions with combined severity factor (CSF) values and enzyme dosages greater than 1.72 and 30 FPU/g water insoluble solid, respectively, were required to obtain an efficient glucan digestibility and a good overall glucose recovery. Up to 81.2% of the sugar in the raw material was recovered for a CSF of 2.25. However, considering overall sugar yield and byproducts concentration, the pretreated material obtained with a CSF of 1.62 can be considered as the most appropriate for SSF experiments using a xylose-utilizing yeast. At these conditions, it could be possible to obtain up to 247 L of ethanol per dry ton of giant bamboo considering hexose and pentose sugars fermentation. This amount could be increased up to 292 L of ethanol per dry ton of giant bamboo with the maximum sugar yield obtained (CSF = 2.25) if the microorganism possesses robust fermentative characteristics as well as a high resistance to pretreatment by-products.
机译:南部非洲地区目前正在建立巨型竹林,可以将其视为生产第二代生物乙醇的潜在木质纤维素原料。在这项研究中,巨大的竹节间材料在酶促水解之前要经过二氧化硫(SO2)浸渍的蒸汽预处理。在固定的SO2浓度为2.5%(w / w液体)后,根据中央复合实验设计(CCD),使用响应表面响应技术研究了温度,停留时间和酸度对总糖回收和副产物形成的影响。浸渍。结果表明,为获得有效的葡聚糖消化率和良好的总葡萄糖回收率,需要分别采用综合严重性因子(CSF)值和酶剂量分别大于1.72和30 FPU / g水不溶性固体的预处理条件。原料中糖的回收率高达81.2%,CSF为2.25。但是,考虑到总糖产量和副产物浓度,CSF为1.62的预处理材料可以被认为是最适合使用利用木糖的酵母进行SSF实验的材料。在这些条件下,考虑到己糖和戊糖的发酵,每干吨巨型竹子可能最多获得247 L乙醇。如果该微生物具有强大的发酵特性以及对预处理副产物的高抗性,则该量可以增加至每干吨巨型竹子292 L乙醇,并获得最大糖产量(CSF = 2.25)。

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