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首页> 外文期刊>Biotechnology Progress >Improvement of Photorhabdus temperata Bioinsecticides Production in Low-Cost Media Through Adequate Fermentation Technology
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Improvement of Photorhabdus temperata Bioinsecticides Production in Low-Cost Media Through Adequate Fermentation Technology

机译：通过适当的发酵技术提高低成本培养基中的光生光菌生物杀虫剂的生产

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摘要

To develop a cost effective process for bioinsecticides production by Photorhabdus temperata, dissolved oxygen (DO) requirements were investigated in both the complex and the optimized media using diluted seawater as a source of micronutrients. By varying DO concentrations, tolerance to hydrogen peroxide was shown to be medium dependant. Indeed, P. temperata cells grown in the complex medium, exhibited higher tolerance than cells grown in the optimized medium (OM). Tolerance to H2O2 was shown to be related to intracellular reactive oxygen species (ROS) accumulation during soya bean meal or glucose assimilation, as shown by flow cytometry analysis. To avoid oxidative stress damages in P. temperata cells cultured in the OM, DO concentration should be constant 50% saturation throughout the fermentation. However, a DO-shift control strategy was demonstrated to be beneficial for P. temperata bioinsecticide production in the complex medium. By using such a strategy biomass, culturability, and oral toxicity reached 16.5 x 10~8, 1.15 x 10~8 cellslmL and 64.2%, respectively, thus was 16.19, 26.37, and 12.2% more than in the cultures carried out at a constant 50% saturation.
机译：为了开发一种经济有效的生产温带光生物杀虫剂的方法，使用稀释的海水作为微量营养素来源，研究了复杂和优化培养基中的溶解氧（DO）需求。通过改变DO浓度，显示出对过氧化氢的耐受性是中等依赖性的。实际上，在复合培养基中生长的温带假单胞菌比在优化培养基（OM）中生长的细胞表现出更高的耐受性。流式细胞仪分析表明，对H2O2的耐受性与豆粕或葡萄糖同化过程中细胞内活性氧（ROS）的积累有关。为了避免在OM中培养的温度假单胞菌细胞受到氧化应激损害，在整个发酵过程中，DO浓度应保持恒定的50％饱和度。但是，DO偏移控制策略被证明对复杂培养基中的温带假单胞菌生物杀虫剂生产是有益的。通过使用这种策略，生物量，可培养性和口服毒性分别达到16.5 x 10〜8、1.15 x 10〜8细胞lmL和64.2％，分别比在恒定的培养条件下分别高16.19、26.37和12.2％。 50％饱和度。

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