• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Detection and Structural Characterization of Insulin Preflbrilar Oligomers Using * Surface Enhanced Raman Spectroscopy
【24h】

Detection and Structural Characterization of Insulin Preflbrilar Oligomers Using * Surface Enhanced Raman Spectroscopy

机译：表面增强拉曼光谱检测和检测胰岛素前纤维状低聚物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In vitro fibril formation typically exhibits a lag phase followed by a rapid elongation phase. Soluble preflbrilar oligomers form as multiple assembly states occur during the lag phase and, after forming a nucleus, rapidly propagate into amyloid aggregates and fibrils. The structure and morphology of amyloid fibrils have been extensively characterized over the last decades, while little is known about the structural organization of the preflbrilar oligomers or their multiple assembly states. The main difficulty in structural characterization of preflbrilar aggregates is their low concentration (pmolar) and their continual reactive conversion. Herein we overcome these difficulties by utilizing Surface-Enhanced Raman Spectroscopy (SERS) with a model amyloid peptide, insulin. SERS is a powerful analytic tool that is able to provide detection of small molecules down to a single-molecule level. Using SERS we found that during the 3 lag phase before the onset of insulin fibril formation, the amount of insulin oligomers increased more than twice after the first hour of incubation under fibrillation conditions (pH 1.6, 65°C) and then slowly decreased with time. The latter finding is kinetically linked to the conversion of the preflbrilar oligomers into fibril species. This study provides valuable new information about the time-dependent structural organization of insulin oligomers and demonstrates the power and potential of SERS for detection and structural characterization of biological specimens present at low concentrations.
机译：体外原纤维形成通常表现出滞后阶段，随后是快速延伸阶段。当在滞后阶段出现多种组装状态时，形成可溶性前纤维状低聚物，并在形成核后迅速繁殖成淀粉样聚集物和原纤维。在过去的几十年中，淀粉样蛋白原纤维的结构和形态已被广泛地表征，而关于前纤维寡聚物的结构组织或它们的多重组装状态知之甚少。前纤维状聚集体的结构表征的主要困难是其低浓度（摩尔）和其连续的反应转化。本文中，我们通过使用表面增强拉曼光谱（SERS）和模型淀粉样肽，胰岛素克服了这些困难。 SERS是功能强大的分析工具，能够提供小分子检测到单分子水平。使用SERS，我们发现在胰岛素原纤维形成开始之前的3个滞后阶段中，在原纤化条件下（pH 1.6、65°C）孵育的第一小时后，胰岛素低聚物的量增加了两倍以上，然后随时间缓慢降低。后一个发现在动力学上与前纤维状低聚物转化为原纤维物种有关。这项研究提供了有关胰岛素低聚物随时间变化的结构组织的有价值的新信息，并证明了SERS对低浓度生物样品的检测和结构表征的功能和潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号