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首页> 外文期刊>Journal of neurosurgical sciences >Characterization and full genome sequencing of a velogenic Newcastle disease virus (NDV) strain Ck/IR/Beh/2011 belonging to subgenotype VII(L)
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Characterization and full genome sequencing of a velogenic Newcastle disease virus (NDV) strain Ck/IR/Beh/2011 belonging to subgenotype VII(L)

机译：培条的特征和完全基因组测序脓性疾病病毒（NDV）菌株CK / IR / BEL / 2011年属于亚因素型VII（L）

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摘要

Avian avulavirus 1, better known as Newcastle disease virus (NDV), causes substantial loss to the poultry industry in many developing countries. In this study we have characterized and fully sequenced the genome of a velogenic NDV strain named Beh (Ck/IR/Beh/2011) that has been used in our lab for a number of challenge and immunological studies over the last few years. This strain was isolated from poultry in the city of Behshahr, Mazandaran Province, Iran after an outbreak reported in the region in 2011. The intracerebral pathogenicity index (ICPI) was 1.8 in one-day-old chicks, characteristic of a velogenic NDV strain. Later, the virus was purified using a sucrose gradient centrifugation and used for next-generation sequencing (NGS). The results showed that the genome length was 15192 bp, similar to those of class II velogenic strains. In addition, the phylogenetic analysis based on the complete F gene showed that the NDV strain Beh has an F protein cleavage site (112)RRQKR down arrow F-117 and belongs to the newly identified subgenotype VII(L). Based on the biological and genetic characterization, NDV strain Beh is now the best documented reference isolate representing the novel subgenotype VII(L) in Iran.
机译：Avian Avulavirus 1，被称为Newcastle疾病病毒（NDV），在许多发展中国家的家禽行业导致大量损失。在这项研究中，我们已经表征并完全测序了我们的实验室中已用于过去几年的一些挑战和免疫学研究的媒体NDV菌株的基因组。该菌株在2011年该地区报告的爆发后，来自Mazhahr城市的家禽，伊朗·伊朗·伊朗。脑内致病性指数（ICPI）在一天大的雏鸡中为1.8，特征是培条NDV菌株。后来，使用蔗糖梯度离心纯化病毒并用于下一代测序（NGS）。结果表明，基因组长度为15192bp，类似于II类测速菌株的BP。此外，基于完整F基因的系统发育分析表明，NDV菌株BEH具有F蛋白质切割位点（112）RRQKR向下箭头F-117，属于新鉴定的亚型型VII（L）。基于生物学和遗传表征，NDV菌株BEH现在是代表伊朗新型亚型VII（L）的最佳记录的参考分离物。

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