首页> 外文期刊>Journal of Helminthology >Morphological and genetic variation of Wuchereria bancrofti microfilariae in carriers in Thailand, Lao PDR and Myanmar: evaluation using Giemsa-stained thick blood films
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Morphological and genetic variation of Wuchereria bancrofti microfilariae in carriers in Thailand, Lao PDR and Myanmar: evaluation using Giemsa-stained thick blood films

机译:泰国,老挝人民主工组织,老挝人民主工组织,老挝人民主工组织,老挝人民主工组织,老挝人和缅甸的形态和遗传变异:使用Giemsa染色血膜评估

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摘要

There is geographical variation in the morphology and genetics of Wuchereria bancrofti, the major cause of human lymphatic filariasis. This study aims to compare morphological and genetic variation of W. bancrofti microfilariae recovered from carriers in Lao PDR, Myanmar and Thailand. Six morphological parameters (body length, cephalic space length and width, length of head to nerve ring, body width at nerve ring, Innenkorper length and number of column nuclei between the cephalic space and nerve ring) were evaluated from microfilariae in Giemsa-stained thick blood films. A portion of the cytochrome c oxidase subunit 1 gene of mitochondrial DNA was sequenced and analysed. Wuchereria bancrofti microfilariae showed a wide variation in their morphology and morphometry among three countries. Phylogenetic analysis confirmed that all microfilariae belonged to W. bancrofti. Higher mutation frequencies were observed in samples from Myanmar, relative to Thailand and Lao PDR. This study highlights the morphological disparities of microfilariae and genetic variability within W. bancrofti among three geographical locations. We found that reported morphometric differences between localities were less clear-cut than previously thought. Further studies are needed to determine the microfilarial periodicity in Lao PDR.
机译:Wuchereria Bancrofti的形态和遗传学的地理变异,人淋巴丝虫病的主要原因。本研究旨在比较从老挝人民主义,缅甸和泰国的运营商中恢复的W.Bancrofti microfilariae的形态和遗传变异。六种形态参数(体长,头部空间长度和宽度,头部长度到神经环,神经环上的体宽,头部korper长度和柱子核的柱核数量从Microotiae评估Giemsa染色厚度血膜。测序并分析线粒体DNA的细胞色素C氧化酶亚基1基因的一部分。 Wuchereria Bancrofti MicroFilariae在三个国家的形态和形态学中显示出广泛的变化。系统发育分析证实,所有Microticariae都属于W. Bancrofti。从缅甸的样品相对于泰国和老挝人民党观察到较高的突变频率。本研究突出了三个地理位置中W.Bancrofti中微胰岛素和遗传变异的形态差异。我们发现报告的情况之间的形态学差异比以前思想的透明度较小。需要进一步的研究来确定老挝人民主工党的微生物周期。

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