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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Effects of double encapsulation and coating on synthetic seed conversion in M.26 apple rootstock
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Effects of double encapsulation and coating on synthetic seed conversion in M.26 apple rootstock

机译：双封装和涂层对M.26苹果砧木合成种子转化的影响

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摘要

Encapsulated vitro-derived apical buds of M.26 apple rootstock (Malus pumila Mil)can be comployed for the formation of the synthetic seed.Satisfactory levels of conversion(plantlets from synthetic seed)canbe achieved if there are adequate(i) rooting induction treatment,(ii)protocol of encapsulation,and (iii) nutritive and environmental conditions.For capsule manufacturing,sodium alginate is largely used;however,this is excessively permeable iwht loss of the nutritive substances(artifical endosperm) and/or dehydration risks during conservation and transport causing detrimental effects on the synthetic seed conversion and on the plantlet's growth.In order to overcome these problems,two experimetns were carried out comparing simple cncapsulation in alginate ith doubl encapsulation,and with encapsulation-coating procedures.The presence of a second layer of alginate(double encapsulation)and of a thin extermal coating laryer over the alginate(encapsulation-coating)did not show any detrimental effects on viability,sprouting and regrowth with the encapsulation-coating rpocedure,whereas the double and simple encapsulation converted less than 40precent of the synthetic seed.THe effect of the addition to the capsule of an anti-microbial substance (Plant Preservative Mixture -PPM)was examined:it did not compromise the conversion of the encapsulated microcuttings sown inex-vitro non-aseptic conditions.
机译：封装的体外衍生的m.26苹果砧木（Malus pumila mil）可以涉及形成合成种子。如果有足够的（i）生根诱导治疗（ii）封装方案，（iii）营养和环境条件。胶囊制造，藻酸钠大部分使用;然而，这是在保护期间过度渗透的营养物质（人工胚乳）和/或脱水风险的损失和运输导致对合成种子转换的有害影响以及植物的生长。为了克服这些问题，进行了两种藻酸盐封装中的简单CNCapsulation，并用封装 - 涂布程序进行了两种模拟。第二层的存在。第二层的存在藻酸盐（双重包封）和藻酸盐上的薄外涂层撕裂（包封涂层）没有显示任何戒律l对封装涂层RPocedure的活力，发芽和再生的影响，而双重和简单的封装转化的合成种子的40℃。添加到抗微生物物质胶囊的效果（植物防腐混合物-PPM检查了）它没有损害封装的微加密的转化播种的羊绒体外非无菌病症。

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